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Investigation of the effects of eugenol and quercetin on bone loss in STZ-NA induced diabetic rats utilizing micro CT
Aycan Baş1,2, Işıl Albeniz2
1Department of Biophysics, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey.
Purpose:
Diabetes Mellitus (DM) is a systemic disease that can effect tissues and their physiological functions at molecular and biochemical levels. Diabetic osteoporosis is one of the chronic diseases of bone metabolism effected by and characterized by augmented risk of osteoporotic fractures and destroying of bone microarchitecture. It was aimed to investigate the alterations in femoral bone structure that may take place as a complication of DM by using the antioxidant properties of eugenol and quercetin, which are phenolic compounds, in streptozotocin nicotinamide (STZ-NA) induced rats as an experimental type 2 DM (T2 DM) model.
Methods:
The antioxidant effect of eugenol and quercetin in case of DM development was determined by GSH ELISA kit. The effect of DM on alterations in bone structure was analyzed by micro CT. BMD, Tb.Bv/Tb.Tv, Tb.N, Tb.Th, Ct.Th, Tb.Sp and SMI data were calculated with the software CTAn.
Results:
Serum GSH levels, Tb.Th and Tb.Bv/Tb.Tv values statistically decreased, and SMI values statistically increased in diabetic group compared with controls. Serum GSH levels in eugenol group were higher than diabetic group, and Tb.Bv/Tb.Tv values in eugenol group were lower than controls. Quercetin group had higher serum GSH levels and Tb.Th values compared with diabetic group, while SMI values were lower in quercetin group compared with diabetic group.
Conclusion:
Eugenol and quercetin were revealed to have antioxidant, antidiabetic and osteoprotective effects on the repair of bone structure in experimental STZ-NA T2 DM model.
Insights
This study shows that eugenol and quercetin possess antioxidant, antidiabetic, and osteoprotective properties. These compounds help repair bone structure in a type 2 diabetes mellitus rat model, mitigating diabetic osteoporosis complications.
Area of Science:
- Biochemistry
- Pharmacology
- Bone Biology
Background:
- Diabetes Mellitus (DM) is a systemic disease impacting tissues at molecular and biochemical levels.
- Diabetic osteoporosis, a complication of DM, increases fracture risk and degrades bone microarchitecture.
- Investigating therapeutic interventions for diabetic bone complications is crucial.
Purpose of the Study:
- To investigate femoral bone structure alterations in a type 2 DM rat model.
- To evaluate the osteoprotective effects of eugenol and quercetin, known antioxidants.
- To assess the potential of these compounds in mitigating DM-induced bone damage.
Main Methods:
- Induced type 2 DM in rats using streptozotocin-nicotinamide (STZ-NA).
- Assessed antioxidant effects via serum glutathione (GSH) levels using ELISA.
- Analyzed bone structure alterations using micro-computed tomography (micro-CT).
Main Results:
- Diabetic rats showed decreased serum GSH, reduced bone thickness (Tb.Th), and lower bone volume/trabecular volume (Tb.Bv/Tb.Tv), with increased structure model index (SMI).
- Eugenol treatment increased serum GSH and improved Tb.Bv/Tb.Tv compared to controls.
- Quercetin treatment elevated serum GSH and Tb.Th, while reducing SMI compared to diabetic rats.
Conclusions:
- Eugenol and quercetin demonstrate significant antioxidant and antidiabetic effects.
- These phenolic compounds exhibit osteoprotective properties, aiding bone structure repair in a T2 DM model.
- Eugenol and quercetin show promise for managing diabetic osteoporosis.
