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Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
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Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
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Overview of Exosomes01:36

Overview of Exosomes

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Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
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Regulation of Angiogenesis and Blood Supply01:24

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Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
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Stem Cell Niche01:26

Stem Cell Niche

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The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
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Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

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The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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Updated: Sep 20, 2025

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
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Exosomes as Carriers for Notch Molecules.

Guya Diletta Marconi1,2, Francesca Diomede3, Oriana Trubiani3

  • 1Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|June 8, 2022
PubMed
Summary

Researchers developed a method to package Delta-like 4 (DLL4) into exosomes using dental pulp stem cells. This facilitates cell-to-cell communication via Notch signaling pathway components, confirmed through analytical methods.

Keywords:
Dental pulp stem cellsExosomesExtracellular vesiclesNotch pathwaySecretome

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Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Area of Science:

  • Biochemistry
  • Cell Biology
  • Biotechnology

Background:

  • Exosomes are key mediators of intercellular communication, transporting proteins and nucleic acids.
  • The Notch signaling pathway is crucial for cell development and differentiation.
  • Delta-like 4 (DLL4) is a vital Notch ligand involved in various biological processes.

Purpose of the Study:

  • To establish a method for packaging Delta-like 4 (DLL4) into exosomes derived from dental pulp stem cells.
  • To confirm the successful internalization and transport of Notch proteins and transcripts via these engineered exosomes.
  • To explore the potential of exosome-mediated delivery of DLL4 for therapeutic applications.

Main Methods:

  • Culturing dental pulp stem cells (DPSCs) to generate exosomes.
  • Isolating and characterizing exosomes from DPSC cultures.
  • Employing molecular biology techniques to package DLL4 into exosomes.
  • Utilizing biochemical and molecular analyses to detect DLL4 protein and transcripts within exosomes and recipient cells.

Main Results:

  • Successfully engineered exosomes containing Delta-like 4 (DLL4) using a dental pulp stem cell model.
  • Confirmed the presence and integrity of DLL4 proteins and transcripts within the generated exosomes.
  • Demonstrated the internalization and transport of DLL4 via exosomes, indicating functional intercellular signaling.

Conclusions:

  • Dental pulp stem cell-derived exosomes can be effectively engineered to carry Delta-like 4 (DLL4).
  • This exosome-based delivery system offers a promising avenue for modulating Notch signaling pathways.
  • The findings support the potential of exosomes as natural nanocarriers for therapeutic ligand delivery.