Biorefinery Cascade Processing for Converting Corncob to Xylooligosaccharides and Glucose by Maleic Acid Pretreatment

Corncob as an abundant and low-cost waste resource has received increasing attention to produce value-added chemicals, it is rich in xylan and regarded as the most preferable feedstock for preparing high value added xylooligosaccharides. The use of xylooligosaccharides as core products can cut costs and improve the economic efficiency in biorefinery. In this study, maleic acid, as a non-toxic and edible acidic catalyst, was employed to pretreat corncob and produce xylooligosaccharides. Firstly, the response surface methodology experimental procedure was employed to maximize the yield of the xylooligosaccharides; a yield of 52.9% (w/v) was achieved with 0.5% maleic acid (w/v) at 155 °C for 26 min. In addition, maleic acid pretreatment was also beneficial to enhance the enzymatic hydrolysis efficiency, resulting in an enzymatic glucose yield of 85.4% (w/v) with a total of 10% solids loading. Finally, a total of 160 g of xylooligosaccharides and 275 g glucose could be produced from 1000 g corncob starting from the maleic acid pretreatment. Overall, a cascade processing for converting corncob to xylooligosaccharides and glucose by sequential maleic acid pretreatment and enzymatic hydrolysis was successfully designed for the corncob wastes utilization.