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Updated: Sep 20, 2025

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Published on: December 28, 2017

Susceptibility Testing of Candida auris Isolates.

Elizabeth M Johnson^1,2, Andrew M Borman^3,4

¹UK National Mycology Reference Laboratory, Public Health England South-West Regional Laboratory, Southmead Hospital, Bristol, UK. Elizabeth.Johnson@nbt.nhs.uk.

Methods in Molecular Biology (Clifton, N.J.)

|June 8, 2022

Summary

Candida auris susceptibility testing guides antifungal selection due to varied resistance profiles. Methods include broth microdilution and gradient strips for accurate treatment choices.

Keywords:

Candida auris MIC Resistance Susceptibility testing

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Area of Science:

Medical Mycology
Antimicrobial Resistance

Background:

Candida auris exhibits diverse antifungal susceptibility profiles across clades and strains.
Innate resistance to fluconazole is common, with cross-resistance to other azoles and antifungal classes also observed.
Multidrug resistance and acquired resistance during treatment are significant clinical concerns.

Purpose of the Study:

To guide the selection of appropriate antifungal agents for Candida auris infections.
To detail reliable methods for assessing Candida auris antifungal susceptibility.

Main Methods:

Reference broth microdilution method for susceptibility testing.
Commercially available gradient strip method for susceptibility testing.

Main Results:

Susceptibility testing is crucial due to variable resistance patterns in Candida auris.
Different clades and strains show distinct responses to antifungal agents.
Multidrug resistance is frequently encountered.

Conclusions:

Accurate susceptibility testing is essential for effective Candida auris treatment.
Standardized methods like broth microdilution and gradient strips aid in clinical decision-making.
Understanding resistance mechanisms is key to combating Candida auris infections.