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An Optimized Electroporation Procedure for Genetic Transformation of Candida auris
Dalal Kasir1, Marwan Osman1,2, Monzer Hamze1
1Laboratoire Microbiologie Santé et Environnement (LMSE), Doctoral School of Sciences and Technology, Faculty of Public Health, Lebanese University, Tripoli, Lebanon.
Abstract:
Candida auris is responsible for recent outbreaks with significant mortality in hospitalized and long-term care patients. As a highly transmissible and multidrug-resistant fungal pathogen, genetic tools are urgently needed for deciphering mechanisms involved in the host-pathogen interactions and potentially identifying new fungal targets for therapeutic development. Here, we provide a reliable transformation protocol based on an efficient electroporation procedure and the use of a mycophenolic acid resistance marker.
Insights
This study introduces a new genetic tool for Candida auris, a dangerous fungus causing hospital outbreaks. The reliable method uses electroporation and a specific marker to help researchers study this multidrug-resistant pathogen.
Area of Science:
- Mycology
- Infectious Diseases
- Molecular Biology
Background:
- Candida auris is a rising global health threat, causing severe infections and high mortality in vulnerable patients.
- Its multidrug resistance and high transmissibility necessitate advanced research tools.
- Understanding host-pathogen interactions is crucial for developing new antifungal therapies.
Purpose of the Study:
- To establish a reliable genetic transformation protocol for Candida auris.
- To facilitate research into the mechanisms of Candida auris pathogenesis.
- To aid in the identification of novel therapeutic targets against this fungal pathogen.
Main Methods:
- Development of an efficient electroporation technique for Candida auris.
- Utilization of a mycophenolic acid resistance marker for genetic selection.
- Optimization of transformation protocols for reproducibility.
Main Results:
- A dependable protocol for genetic manipulation of Candida auris was successfully established.
- The optimized electroporation method demonstrated high efficiency.
- The mycophenolic acid resistance marker proved effective for selecting transformed cells.
Conclusions:
- The developed genetic transformation protocol provides a vital tool for Candida auris research.
- This advancement will accelerate studies on host-pathogen interactions and drug resistance.
- Enables future development of targeted antifungal strategies against Candida auris.
