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Examining Neutrophil-Candida auris Interactions with Human Neutrophils Ex Vivo
Chad J Johnson1, Jeniel E Nett2,3
1Department of Medicine, University of Wisconsin, Madison, WI, USA.
Abstract:
Neutrophils play a key role in controlling invasive fungal infections. These phagocytes engage and kill fungal pathogens through a variety of effector mechanisms. Here, we describe how to isolate human neutrophils for ex vivo study of neutrophil-Candida auris interactions. We detail assays to measure fungal killing, phagocytosis, and reactive oxygen species production.
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