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Mouse Gastrointestinal Colonization Model for Candida auris.
Stylianos C Simantirakis1, Donna M MacCallum2
1Aberdeen Fungal Group, Institute of Medical Sciences, School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, UK.
Methods in Molecular Biology (Clifton, N.J.)
|June 8, 2022
Summary
This study presents a new mouse model for tracking persistent gut colonization by the fungus Candida auris. This model helps researchers understand how Candida auris spreads within the host.
Area of Science:
- Mycology
- Infectious Diseases
- Immunology
Background:
- Animal models are crucial for studying host-pathogen interactions and disease.
- Candida auris is an emerging fungal pathogen causing significant health concerns.
- Understanding Candida auris gut colonization is vital for developing effective treatments.
Purpose of the Study:
- To establish and validate an effective mouse model for investigating persistent gut colonization by Candida auris.
- To assess the dissemination potential of Candida auris from the gut to other organs in immunocompetent mice.
Main Methods:
- Mice were orally inoculated with Candida auris via gavage.
- Fungal stool burdens were monitored daily to quantify gut colonization.
- Fungal burdens in spleen, liver, kidneys, lungs, heart, and caecum were determined post-mortem to assess dissemination.
Main Results:
- The developed model effectively demonstrated persistent gut colonization by Candida auris in mice.
- The study quantified fungal burdens in various organs, providing insights into potential dissemination.
Conclusions:
- This mouse model offers a valuable tool for studying Candida auris gut colonization and host-pathogen dynamics.
- Further research using this model can elucidate C. auris pathogenesis and inform therapeutic strategies.
