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Evaluating Nanoparticle Hydrophobicity Using Analytical Membrane Hydrophobic Interaction Chromatography.

Neil Taylor1, Wanli Ma2, Adam Kristopeit2

  • 1Department of Chemical Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802, United States.

Analytical Chemistry
|June 8, 2022
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Summary

This study introduces membrane hydrophobic interaction chromatography (MHIC) to measure nanoparticle hydrophobicity. This new method accurately assesses larger nanoparticles and viruses for applications in drug delivery and gene therapy.

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biotechnology

Background:

  • Nanoparticle hydrophobicity is crucial for stability, adhesion, and transport in suspensions.
  • Existing methods struggle with larger nanoparticles and viruses (>100 nm), vital for drug delivery and gene therapy.
  • A need exists for a versatile method to quantify hydrophobicity in diverse nanoparticle systems.

Purpose of the Study:

  • To develop and validate a novel membrane hydrophobic interaction chromatography (MHIC) method for assessing nanoparticle hydrophobicity.
  • To evaluate the applicability of MHIC for both model proteins and complex nanoparticles, including viruses.
  • To demonstrate the method's utility in various conditions, such as in water and with surfactants.

Main Methods:

  • Utilized a polyvinylidene fluoride membrane (5.0 μm pore size) as the stationary phase in a membrane hydrophobic interaction chromatography (MHIC) setup.
  • Validated the method using a series of model proteins, comparing results with established hydrophobicity data.
  • Applied MHIC to assess the hydrophobicity of diverse nanoparticles, including a live attenuated viral vaccine, in different media.

Main Results:

  • Experimental data for model proteins showed strong agreement with literature values for hydrophobicity.
  • MHIC successfully quantified the hydrophobicity of various nanoparticles, including a viral vaccine.
  • The method proved effective in both aqueous solutions and in the presence of different surfactants.

Conclusions:

  • Membrane hydrophobic interaction chromatography (MHIC) offers a reliable and accessible approach for evaluating nanoparticle hydrophobicity.
  • The developed MHIC method is suitable for large nanoparticles and viruses, addressing limitations of previous techniques.
  • This technique is compatible with standard liquid chromatography systems, offers rapid analysis (<20 min), and avoids organic solvents, preserving biological nanoparticle integrity.