Central neurotoxic effects of guanethidine: altered serotonin and enkephalin neurons within the area postrema

This study reveals that the 5-HT and enkephalin cell body population of the area postrema (AP) is dramatically reduced via exposure to the peripherally circulating neurotoxin guanethidine. Efforts to restore cell body numbers to control levels, with colchicine and monoamine oxidase inhibitors, subsequent to guanethidine treatment are ineffective. However, 5-HT and enkephalin immunostaining of surrounding bulbar nuclei appears normal, implying that the neurotoxic effect of guanethidine is restricted to the AP. In addition, gamma-aminobutyric acid and neurotensin immunostaining within the AP appears normal, subsequent to guanethidine treatment, suggesting that the neurotoxic effect is restricted to specific AP cell populations.