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Protein Dynamics in Living Cells

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Updated: Sep 20, 2025

Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins

Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins

Published on: June 15, 2018

Advanced functional fluorescent probes for cell plasma membranes.

Mayeul Collot¹, Sonia Pfister¹, Andrey S Klymchenko¹

¹Laboratoire de Bioimagerie et Pathologies, UMR 7021, CNRS/Université de Strasbourg, 74 route du Rhin, 67401, Illkirch-Graffenstaden, France.

Current Opinion in Chemical Biology

|June 8, 2022

Summary

Advanced fluorescent probes offer new ways to image the plasma membrane (PM) for cell studies. These molecular tools enable advanced sensing and super-resolution imaging of cellular structures.

Keywords:

Fluorescence microscopy Fluorescent probes Lipid probes Plasma membrane probes Probing cell surface SMLM STED Super resolution

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Area of Science:

Biochemistry
Cell Biology
Molecular Imaging

Background:

Fluorescence imaging of the plasma membrane (PM) is crucial for understanding cell biology.
Molecular fluorescent probes are key tools for visualizing and analyzing cellular structures and dynamics.
Current probes enable cell segmentation, membrane organization studies, and vesicle tracking.

Purpose of the Study:

To review recent advancements in fluorescent probes for plasma membrane (PM) imaging.
To highlight novel functions of rationally designed PM probes beyond basic staining.
To cover applications in chemical/biophysical sensing and super-resolution imaging.

Main Methods:

Review of recent scientific literature on fluorescent PM probes.
Analysis of molecular design strategies for enhanced probe functionality.
Categorization of probes based on sensing capabilities and imaging resolution.

Main Results:

Molecular design has significantly advanced fluorescent PM probe capabilities.
New probes offer enhanced specificity and functionality for PM studies.
Recent probes facilitate advanced chemical and biophysical sensing.
Novel probes are enabling super-resolution imaging of the PM.

Conclusions:

Rationally designed fluorescent PM probes represent a significant leap in cellular imaging.
These advanced probes are powerful tools for chemical/biophysical sensing and super-resolution microscopy.
Future developments promise even greater insights into PM organization and dynamics.