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Related Experiment Video

Updated: Sep 20, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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Rapid, adaptable and sensitive Cas13-based COVID-19 diagnostics using ADESSO.

Beatrice Casati1,2, Joseph Peter Verdi1,3, Alexander Hempelmann3

  • 1Division of Immune Diversity, Department of Immunology and Cancer, German Cancer Research Centre (DKFZ), 69120, Heidelberg, Germany.

Nature Communications
|June 8, 2022
PubMed
Summary

A new diagnostic test, ADESSO, accurately detects SARS-CoV-2, the virus causing COVID-19, in one hour. This fast, sensitive, and adaptable method offers a promising solution for point-of-care testing and variant detection.

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Area of Science:

  • Molecular diagnostics
  • Viral detection technologies
  • Point-of-care testing

Background:

  • Current COVID-19 diagnostic methods like PCR and antigen tests have limitations in sensitivity and adaptability to emerging SARS-CoV-2 variants.
  • The need for rapid, accurate, and flexible diagnostic tools is critical for pandemic control and managing evolving viral strains.

Purpose of the Study:

  • To develop and validate ADESSO (Accurate Detection of Evolving SARS-CoV-2 through SHERLOCK Optimization), a novel diagnostic test for SARS-CoV-2.
  • To assess ADESSO's performance in terms of speed, sensitivity, specificity, and adaptability for detecting evolving SARS-CoV-2 strains.

Main Methods:

  • Development of ADESSO utilizing Cas13 enzyme technology and SHERLOCK (Specific High Sensitivity Enzymatic Reporter UnLOCKing).
  • Evaluation of ADESSO using an extensive panel of clinical samples, comparing its performance against RT-qPCR and antigen tests.
  • Assessment of the test's ability to function without sophisticated equipment and within a one-hour timeframe.

Main Results:

  • ADESSO demonstrated high sensitivity and specificity comparable to RT-qPCR on extracted RNA.
  • For unextracted samples, ADESSO showed higher sensitivity than existing antigen tests.
  • The test successfully diagnosed COVID-19 patients within one hour, requiring minimal equipment.

Conclusions:

  • ADESSO is a fast, sensitive, and cost-effective diagnostic method suitable for point-of-care settings.
  • The test's design allows for rapid adaptation to detect new SARS-CoV-2 variants, addressing a key limitation of current diagnostics.