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Proteomic Profiling of Cryoglobulinemia
Peng Liu1, Jianqiang Wu1, Dandan Sun2
1Medical Research Center, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Frontiers in Immunology
|June 9, 2022
Summary
Researchers identified novel protein biomarkers for cryoglobulinemia (CGE) using a TMT-PRM-ELISA workflow. Apolipoprotein A1 (APOA1) and PCSK9 levels were elevated in CGE patients, suggesting cholesterol metabolism
Area of Science:
- Proteomics
- Biomarker Discovery
- Immunology
Background:
- Cryoglobulinemia (CGE) is a condition characterized by the presence of cryoglobulins.
- Identifying specific biomarkers for CGE is crucial for diagnosis and understanding disease pathogenesis.
- Previous research has not fully elucidated the proteomic landscape associated with CGE.
Purpose of the Study:
- To identify and validate novel serum protein biomarkers for cryoglobulinemia (CGE).
- To investigate the role of cholesterol metabolism proteins in CGE pathogenesis.
- To establish a robust workflow for CGE biomarker discovery.
Main Methods:
- Quantitative proteomics using tandem mass tag (TMT) labeling was employed to compare serum samples from CGE patients and disease controls (DC).
- Ingenuity pathway analysis (IPA) was used for functional annotation of differentially expressed proteins.
- Biomarker candidates were validated using parallel reaction monitoring (PRM) and enzyme-linked immunosorbent assay (ELISA).
Main Results:
- A total of 1004 proteins were identified, with 109 differentially expressed between CGE and DC groups.
- Differentially expressed proteins were associated with hepatic fibrosis, inflammation, and cholesterol/lipid transport.
- Apolipoprotein A1 (APOA1) and proprotein convertase subtilisin/kexin type-9 (PCSK9) concentrations were significantly increased in CGE patients compared to healthy controls.
Conclusions:
- The study presents the first TMT-PRM-ELISA workflow for CGE biomarker identification and validation.
- Elevated levels of APOA1 and PCSK9 in CGE patients highlight the involvement of cholesterol metabolism in the disease.
- These findings advance CGE pathogenesis research and biomarker discovery efforts.
