Related Experiment Video
Updated: Sep 20, 2025
Author Spotlight: Improved Nucleofection for High-Efficiency Gene Delivery in Murine Subventricular Zone-Derived Neural Stem Cell Cultures
Published on: June 14, 2024
Protocol for isolating adult pituitary stem/progenitor cells in mice
Aran Shintani1,2, Masashi Higuchi1,2
1Laboratory of Veterinary Biochemistry, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-minami, Tottori-shi, Tottori 680-8553, Japan.
Abstract:
Hormone-producing cells in the anterior lobe (AL) of the pituitary gland are important for growth and reproduction, but it has been challenging to isolate their source: the pituitary stem/progenitor cells. Here, we present a protocol for isolating adult pituitary stem/progenitor cells (APSCs) in mice. We describe dissociation and culture of AL cells, followed by the assessments of stemness marker expression and the differentiation capacity. This protocol enables separation of APSCs based on their cell adhesion properties with nearly 100% purity. For complete details on the use and execution of this protocol, please refer to Shintani and Higuchi (2021).
