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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Dolabrane-Type Diterpenoids with Immunosuppressive Activity from Koilodepas hainanense
Yu Ren1,2, Xiao Tong1,2, Ye Zhao3,2
1University of Chinese Academy of Science, No. 19A Yuquan Road, Beijing 100049, People's Republic of China.
Abstract:
Thirteen new dolabrane-type diterpenoids, koilodenoids A-M (1-13), including a chlorinated congener (2), along with six known analogues, were isolated from Koilodepas hainanense. The structures were determined by analysis of spectroscopic data, ECD spectra, and X-ray crystallographic studies. The absolute configuration of C-15 in the 15,16-diol unit of compounds 4 and 5 was established by using the dimolybdenum tetraacetate [Mo2(AcO)4]-induced ECD method. Compounds 4, 7, 16, 17, and 19 showed moderate to significant immunosuppressive activities against the proliferation of T and B lymphocytes in vitro, with compound 16 being the most potent (IC50 0.86 and 0.29 μM, respectively).
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