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Updated: Sep 20, 2025
Measuring Skeletal Muscle Thermogenesis in Mice and Rats
Published on: July 27, 2022
Activation of thermogenesis pathways in testis of diet-induced obesity mice
Hucheng Qing1, Juan Hu2, Huheng Fu3
1Department of Biochemistry and Molecular Biology, Medical School, Southeast University, #87 Dingjiaqiao Road, Gulouqu, Nanjing, Jiangsu Province 210009, China; Environmental Health Risk Assessment and Prevention Engineering Center of Ecological Aluminum Industry Base of Youjiang Medical University for Nationalities, Baise, Guangxi 533000, China.
Abstract:
High-fat diet (HFD) induced obesity (DIO) has been shown impacts on metabolism, hormonal profile, male fertility, and spermatogenesis. We employed genome-wide transcriptional analysis on the testis of diet induced obesity (DIO) and normal chow (NC) C57BL/6 J male mice to search genes regulated by obesity in testis. Both blood glucose and lipids contents significantly increased in DIO mice after 8 weeks fat-rich feeding. RNA-seq analysis revealed 371 down-regulated and 460 up-regulated transcripts in DIO group comparing to NC group. Chromosome 3, 4, 9, 16, and 18 were significantly more active, while chromosome 5, 10, and 19 were significantly more inactive after 8-week fat-diet feeding. Wilcoxon enrichment analysis discovered that the thermogenesis pathway (KEGG) was significantly enriched in the testis of DIO group (with 8 enriched up-regulated genes: Smarca2, Adcy3, Atp5pb, Creb1, Gnas, Rps6kb2, Upcrc1 and Dpf1). Real-time PCR further confirmed that Smarca2 and Atp5pb were upregulated in the testis of DIO mice. These finding implied that diet-induced thermogenesis pathways could be altered in the testis of DIO mice.
