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[KMT2A Activates Akt/mTOR Signaling Pathway Through LncRNA-HOTAIR to Influence the Biological Behavior of AML Cells]
Yun Huang1, Ai-Li Zhang2, Rui Su1
1Department of Hematology, The First Affiliated Hospital of Xiamen University, Xiamen 361003, Fujian Province, China.
Objective:
To investigate the expression of lysine methyltransferase 2A (KMT2A) in acute myeloid leukemia (AML) cells and its molecular mechanism affecting the proliferation of AML cells.
Methods:
Co-immunoprecipitation assay was used to detect the binding of KMT2A to long non-coding RNA-HOX transcript antisense RNA (lncRNA-HOTAIR). AML cell proliferation was detected by 5-ethynyl-2'-deoxyuridine (EdU) assay.
Results:
The PCR amplification signal of KMT2A group was significantly stronger than that of the negative control group and IgG group (P<0.01). Compared with the negative control group and KMT2A-OE + lncRNA-HOTAIR-KD group, the ratio of EdU+ cells in both KMT2A-OE group and lncRNA-HOTAIR-OE group significantly increased (P<0.01). Compared with negative control group, the ratio of EDU+ cells in KMT2A-KD group and lncRNA-HOTAIR-KD group significantly decreased (P<0.01), the expression levels of p-Akt and p-mTOR in both KMT2A-OE group and lncRNA-HOTAIR-OE group significantly increased (P<0.01).
Conclusion:
KMT2A can interact with lncRNA-HOTAIR to promote the activation of Akt/mTOR signaling pathway, thus promoting the proliferation of AML cells.
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