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Related Experiment Video

Updated: Sep 20, 2025

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Glioblastoma Stem-like Cell Detection Using Perfusion and Diffusion MRI.

Tanguy Duval1, Jean-Albert Lotterie1,2, Anthony Lemarie3,4

  • 1ToNIC, Toulouse NeuroImaging Center, Université de Toulouse, Inserm, UPS, 31000 Toulouse, France.

Cancers
|June 10, 2022
PubMed
Summary

Glioblastoma stem-like cells (GSCs) drive tumor aggressiveness. Imaging markers like low Apparent Diffusion Coefficient (ADC) and high relative Cerebral Blood Volume (rCBV) can help locate these cells for improved treatment targeting.

Keywords:
FLAIRMRIdiffusionglioblastomaperfusionstem cellssurgery

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Area of Science:

  • Neuro-oncology
  • Medical Imaging
  • Cancer Stem Cell Biology

Background:

  • Glioblastoma (GBM) treatment involves surgery and chemo-radiation, but most patients relapse within a year.
  • Relapse is often linked to radio-resistant glioblastoma stem-like cells (GSCs) in the non-contrast-enhanced (NCE) peritumoral region.
  • The imaging characteristics of these infiltrative GSCs remain largely unstudied.

Purpose of the Study:

  • To investigate the magnetic resonance (MR) imaging signature of infiltrative glioblastoma stem-like cells.
  • To determine if MR imaging can help localize GSCs within the peritumoral NCE region.
  • To correlate imaging findings with GSC density and aggressiveness.

Main Methods:

  • A clinical trial involving 16 glioblastoma patients.
  • Preoperative diffusion and perfusion MRI to measure Apparent Diffusion Coefficient (ADC) and relative Cerebral Blood Volume (rCBV).
  • Image-guided surgical biopsies from the FLAIR hyperintensity region, followed by GSC quantification and in vitro cultivation.

Main Results:

  • Low ADC values predicted faster GSC neurosphere formation in vitro.
  • Higher concentrations of GSCs were found in regions exhibiting high rCBV.
  • ADC and rCBV show potential as imaging biomarkers for GSC localization.

Conclusions:

  • Glioblastoma stem-like cells play a critical role in tumor aggressiveness.
  • Peritumoral areas with low ADC or high rCBV are indicative of GSC presence.
  • These findings support prioritizing surgical removal and radiotherapy targeting of these specific peritumoral sites.