Analysis for carboxyhemoglobin by gas chromatography and multicomponent spectrophotometry compared

Area of Science:

• Clinical Chemistry
• Analytical Chemistry

Background:

• Carboxyhemoglobin (HbCO) is a critical biomarker in clinical diagnostics.
• Accurate measurement of HbCO is essential for patient diagnosis and monitoring.

Purpose of the Study:

• To compare the accuracy of gas chromatography (GC) for measuring carboxyhemoglobin (HbCO) against multicomponent spectrophotometric analysis (MCA).
• To assess the linearity and stability of the GC method for HbCO determination.

Main Methods:

• Comparison of HbCO measurements obtained by GC and MCA across various concentration ranges.
• Evaluation of method linearity from 0.15% to 30% HbCO.
• Assessment of sample stability after storage at 4°C for two months.

Main Results:

• Good correlation between GC and MCA was observed for HbCO ranges above 1.5% (r = 0.93–0.99).
• Lower correlation was noted in the clinically normal range of 0–1.5% (r = 0.87).
• The GC method demonstrated linearity from 0.15% to 30% HbCO, and samples were stable for two months at 4°C.

Conclusions:

• Gas chromatography provides a reliable and accurate method for quantifying carboxyhemoglobin, particularly at higher concentrations.
• The GC method is suitable for clinical use due to its linearity and the stability of blood samples under refrigerated storage.