Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Two monoclonal-based assays for carcinoembryonic antigen compared.

R A Felder, R H MacMillan, D E Bruns

    Clinical Chemistry
    |May 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Transient depletion of macrophages alters local inflammatory response at the site of disc herniation in a transgenic mouse model.

    Osteoarthritis and cartilage·2023
    Same author

    Dopaminergic Immunofluorescence Studies in Kidney Tissue.

    Methods in molecular biology (Clifton, N.J.)·2017
    Same author

    Common variants of the G protein-coupled receptor type 4 are associated with human essential hypertension and predict the blood pressure response to angiotensin receptor blockade.

    The pharmacogenomics journal·2015
    Same author

    [The actual issues of measurement and presentation of results of albumin excretion with urine].

    Klinicheskaia laboratornaia diagnostika·2012
    Same author

    [Current issues in measurement and reporting of urinary albumin excretion].

    Annales de biologie clinique·2010
    Same author

    D1 dopamine receptor hyperphosphorylation in renal proximal tubules in hypertension.

    Kidney international·2006

    Two new double-monoclonal antibody methods for carcinoembryonic antigen (CEA) show similar precision and specificity for tumor-associated CEA. These assays offer improved diagnostic accuracy compared to older antiserum methods.

    Area of Science:

    • Clinical Chemistry
    • Immunochemistry
    • Oncology Diagnostics

    Background:

    • Conventional antiserum methods for carcinoembryonic antigen (CEA) can yield clinically divergent results.
    • Monoclonal antibody-based assays offer potential improvements in diagnostic specificity and accuracy.
    • Evaluating new commercial CEA assays is crucial for clinical decision-making.

    Purpose of the Study:

    • To assess the performance of two commercial double-monoclonal antibody methods for CEA detection.
    • To compare the precision, accuracy, and specificity of Abbott Diagnostics and Roche Diagnostic Systems CEA assays.
    • To evaluate the clinical utility of these novel assays in cancer patient populations.

    Main Methods:

    • Performance evaluation of Abbott and Roche double-monoclonal antibody CEA assays.

    Related Experiment Videos

  • Precision assessment using total and within-run coefficients of variation (CVs) over 20 days.
  • Correlation analysis of assay results in patients with CEA-secreting carcinomas and serial cancer patient samples.

    • Main Results:

    • Both assays demonstrated similar precision, with total CVs ranging from 6-16% and within-run CVs from 8-15%.
    • Mean CEA concentrations were consistently lower with the Roche assay compared to the Abbott assay.
    • Strong correlations were observed between the two assays (r = 0.88 in carcinomas, r = 0.92 in serial samples).
    • Both methods exhibited approximately 10% false-positive rates in non-malignant conditions.

    Conclusions:

    • The two double-monoclonal antibody CEA assays exhibit comparable precision and significant correlation.
    • These methods appear more specific for tumor-associated CEA than previous polyclonal antibody-based assays.
    • The findings support the clinical utility of these advanced CEA assays for cancer diagnosis and monitoring.