Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

328
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
328
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

5.6K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
5.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Desmoplastic myxoid tumor, SMARCB1-mutant: a case with late recurrence after long-term follow-up.

Free neuropathology·2026
Same author

Distinct molecular subgroups in pediatric and young-onset meningiomas require age-adapted risk stratification.

Nature communications·2026
Same author

Hetairos is a histology-based artificial intelligence model for predicting central nervous system tumor methylation subtypes.

Nature cancer·2026
Same author

Are pediatric central nervous system tumors on the rise in France? A population-based 21-year retrospective study.

Cancer epidemiology·2026
Same author

Glioneuronal Tumour With Neurocytic Differentiation (GNTN): a Molecularly Defined Tumour Type Formerly Referred to as Extraventricular Neurocytoma.

Neuropathology and applied neurobiology·2026
Same author

Trans orbital eyebrow approach for selective resections in mesial temporal lobe epilepsy: Technical aspects and preliminary case series.

Brain & spine·2026

Related Experiment Video

Updated: Sep 20, 2025

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
10:35

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

8.0K

Comparative Study Between a Customized Bimodal Endoscope and a Benchtop Microscope for Quantitative Tissue Diagnosis.

Hussein Mehidine1, Bertrand Devaux2,3,4, Pascale Varlet2,5,6

  • 1Université Paris-Saclay, CNRS/IN2P3, IJCLab, Orsay, France.

Frontiers in Oncology
|June 10, 2022
PubMed
Summary

A new two-photon fluorescence fibered endomicroscope aids surgeons in distinguishing brain tumors from healthy tissue during surgery. This advanced imaging tool provides real-time diagnostic information, improving tumor boundary delineation and potentially reducing recurrence risk.

Keywords:
brain tumorsendomicroscopefluorescencefluorescence lifetimespectroscopy

More Related Videos

Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation
09:42

Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation

Published on: August 26, 2014

18.8K
Flexible Colonoscopy in Mice to Evaluate the Severity of Colitis and Colorectal Tumors Using a Validated Endoscopic Scoring System
15:49

Flexible Colonoscopy in Mice to Evaluate the Severity of Colitis and Colorectal Tumors Using a Validated Endoscopic Scoring System

Published on: October 16, 2013

32.0K

Related Experiment Videos

Last Updated: Sep 20, 2025

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
10:35

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

8.0K
Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation
09:42

Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation

Published on: August 26, 2014

18.8K
Flexible Colonoscopy in Mice to Evaluate the Severity of Colitis and Colorectal Tumors Using a Validated Endoscopic Scoring System
15:49

Flexible Colonoscopy in Mice to Evaluate the Severity of Colitis and Colorectal Tumors Using a Validated Endoscopic Scoring System

Published on: October 16, 2013

32.0K

Area of Science:

  • Biomedical Engineering
  • Neuroscience
  • Surgical Oncology

Background:

  • Surgical removal is the standard for brain tumors, but differentiating tumor margins from healthy tissue is challenging.
  • Infiltrating tumor residuals increase recurrence risk and decrease patient survival.
  • Accurate intraoperative delimitation of tumor boundaries is crucial for improving surgical outcomes.

Purpose of the Study:

  • To develop and validate a two-photon fluorescence fibered endomicroscope for real-time, intraoperative brain tumor diagnosis.
  • To assess the endomicroscope's ability to provide reliable diagnostic information for distinguishing healthy from cancerous brain tissue.
  • To confirm that the developed endoscope can replicate diagnostic indicators from an established optical database.

Main Methods:

  • Development of a two-photon fluorescence fibered endomicroscope for intraoperative use.
  • Construction of an optical database characterizing healthy and tumor brain tissues using endogenous fluorescence.
  • Comparison of spectrally and time-resolved fluorescence signals from the endoscope with a standard multiphoton microscope using 46 fresh brain tissue samples.

Main Results:

  • The endoscope demonstrated higher excitation efficiency and endogenous fluorescence signal collection ability compared to the reference setup.
  • Similar molecular ratios and fluorescence lifetime distributions were obtained from both the endoscope and the reference microscope.
  • The spectral discrimination ability of the bimodal endoscope was validated, confirming its reliability for tissue differentiation.

Conclusions:

  • The developed bimodal fibered endoscope efficiently excites and collects signals, providing high-quality, exploitable data for intraoperative discrimination of human brain tissues.
  • This technology offers a promising tool for neurosurgeons to improve tumor boundary delineation and surgical precision.
  • The validated performance represents a significant preliminary step towards multimodal imaging for enhanced brain tumor surgery.