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Macrophage Fate Mapping.

Yingzheng Xu1, Patricia R Schrank1, Jesse W Williams1

  • 1Center for Immunology, Department of Integrative Biology & Physiology, University of Minnesota, Minneapolis, Minnesota.

Current Protocols
|June 10, 2022
PubMed
Summary

Fate mapping techniques allow researchers to track the origins and turnover of macrophages and monocytes in tissues. These methods are crucial for understanding how these cells contribute to tissue homeostasis and disease progression.

Keywords:
fate mappingmacrophage originmononuclear phagocytesreporter micetamoxifen

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Area of Science:

  • Immunology
  • Cell Biology
  • Developmental Biology

Background:

  • Tissue-resident macrophages are vital for homeostasis and immune surveillance, originating from embryonic progenitors or recruited monocytes.
  • Understanding macrophage ontogeny and cellular turnover is key to deciphering their roles in tissue health and disease.

Purpose of the Study:

  • To outline and discuss various myeloid cell fate mapping approaches for tracking macrophages in mice.
  • To detail constitutive and inducible labeling strategies for both adult and embryonic tissues.

Main Methods:

  • Utilizing fate mapping (lineage tracing) techniques based on Cre-recombinase-mediated gene expression for reporter systems.
  • Employing temporal deployment of fate mapping in conjunction with developmental stages, disease onset, or inflammation resolution.
  • Integrating myeloid fate mapping with advanced technologies like single-cell RNA-sequencing and spatial imaging.

Main Results:

  • Myeloid cell fate mapping has facilitated significant discoveries in myeloid cell ontogeny, tissue residency, and monocyte behavior in disease models.
  • This approach enables precise tracking of cell origins and dynamics within various tissue environments.

Conclusions:

  • Fate mapping is an indispensable tool for investigating macrophage biology, offering insights into their development, maintenance, and function in health and disease.
  • The described protocols provide a foundation for researchers to explore myeloid cell dynamics in mice using established and emerging fate mapping strategies.