Tracking the Stepwise Formation of a Water-Soluble Fluorescent Tb₁₂ Cluster for Efficient Doxorubicin Detection

Doxorubicin (DOX) is an anthraquinone drug used for the efficient treatment of a variety of tumors in human beings. Unfortunately, its poor biodegradability causes incomplete metabolism in the body. Therefore, it is of great significance to synthesize a sensitive and selective material for DOX detection. In this paper, we report a water-soluble Tb₁₂ cluster and track its step-by-step formation (L → Tb₁L₁ → Tb₂L₁ → Tb₂L₂ → Tb₃L₂ → Tb₄L₂ → Tb₁₂L₆). Tb₁₂ can be used to determine the presence of DOX, which quenches the luminescence of the Tb₁₂ aqueous solution, and the detection limit can reach 13 nM (K_SV = 8.7 × 10⁵ M^-1). Tb₁₂ has advantages of high sensitivity and high selectivity for the detection of DOX in a simulated environment of human urine and serum.