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Updated: Sep 20, 2025

Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform
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In vitrocirculation model driven by tissue-engineered dome-shaped cardiac tissue.

Tetsutaro Kikuchi1, Katsuhisa Matsuura1, Tatsuya Shimizu1

  • 1Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns), 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.

Biofabrication
|June 10, 2022
PubMed
Summary

Researchers developed a novel dome-shaped cardiac tissue from stem cells that mimics the human heart's pumping function. This innovative cardiac tissue model can circulate culture medium, simulating the circulatory system for drug testing.

Keywords:
cardiomyocytecell sheet technologydrug screeninginduced pluripotent stem cellorgan on a chiptissue engineering

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Area of Science:

  • Cardiovascular Research
  • Stem Cell Biology
  • Biomaterials Engineering

Background:

  • Three-dimensional cardiac tissues from cardiomyocytes are used for in vitro drug evaluation.
  • Existing models lack the ability to simulate the human circulatory system.
  • A functional, heart-like pump model is needed for advanced in vitro studies.

Purpose of the Study:

  • To create a dome-shaped cardiac tissue using cell sheet stacking that mimics the heart's pump function.
  • To evaluate the potential of this cardiac tissue to simulate the human circulatory system.
  • To assess its utility in in vitro drug evaluation and as a biological pump.

Main Methods:

  • Human induced pluripotent stem cells were differentiated into cardiomyocytes.
  • Cardiomyocyte and fibroblast cell sheets were stacked and inflated into a dome shape.
  • Cardiac tissue beating, stroke volume, pressure, and flow rate were measured.
  • Pressure-volume diagrams were generated under varying fluidic resistance.

Main Results:

  • A dome-shaped cardiac tissue (8 mm diameter) achieved autonomous beating and pump function.
  • Stroke volume reached 21 ± 8.9 μl by day 21 and responded to stimulants.
  • Pulsatile pressure reached 0.33 ± 0.048 mmHg, driving a flow rate of ~1 μl/s.
  • The model generated pressure-volume diagrams, demonstrating its functional capacity.

Conclusions:

  • The fabricated dome-shaped cardiac tissue successfully mimics heart pump function.
  • This model can circulate culture medium, simulating the human circulatory system.
  • It holds potential for use in multi-organ chips and for enhanced in vitro drug evaluation.