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Genomic Epidemiology of MBL-Producing Pseudomonas putida Group Isolates in Poland

Paweł Urbanowicz1, Radosław Izdebski2, Marta Biedrzycka2

  • 1Department of Molecular Microbiology, National Medicines Institute, ul. Chełmska 30/34, 00-725, Warsaw, Poland. p.urbanowicz@nil.gov.pl.

Infectious Diseases and Therapy
|June 10, 2022
PubMed
Abstract

Insights

Metallo-beta-lactamase (MBL)-producing Pseudomonas putida group (MPPP) isolates in Poland showed high diversity, with three main international lineages identified. These lineages carry MBL-encoding integrons, contributing to antimicrobial resistance in these opportunistic pathogens.

Area of Science:

  • Microbiology
  • Genomics
  • Antimicrobial Resistance

Background:

  • Pseudomonas putida group are opportunistic pathogens and reservoirs of antimicrobial resistance (AMR) genes, notably metallo-β-lactamases (MBLs).
  • Understanding the population structure and genetic basis of MBL production in this group is crucial for infection control.

Purpose of the Study:

  • To perform molecular and genomic characterization of MBL-producing P. putida (MPPP) group isolates from Poland.
  • To identify successful genotypes, population structures, and MBL-encoding integrons within these isolates.

Main Methods:

  • Whole-genome sequencing (WGS) of 59 MPPP isolates collected nationwide (2003-2016).
  • Species identification, multi-locus sequence typing (MLST), SNP-based phylogenetic analysis, resistome determination, and susceptibility testing were conducted.

Main Results:

  • The study identified 12 Pseudomonas species, with P. alloputida, P. monteilii, and P. asiatica predominating.
  • Three main international clones were identified (P. alloputida ST69, P. monteilii ST95, P. asiatica ST15), carrying VIM-like MBLs (mostly VIM-2) encoded by diverse class 1 integrons.
  • Multiple isolates exhibited extensive drug resistance, with plasmids of IncP-9, IncP-2, and pMOS94-like types dominating.

Conclusions:

  • This comprehensive analysis reveals significant diversity within MPPP isolates in Poland.
  • Three international lineages of MPPP harbor MBL-encoding structures, contributing to their adaptability and spread.
Keywords:
CarbapenemaseClonal spreadGenomicsMBLMetallo-β-lactamasePhylogenetic analysisPseudomonas putida groupVIM

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