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A high-throughput HPLC method for simultaneous quantification of pyrethroid and pyriproxyfen in long-lasting
Kyle J Walker1, Christopher T Williams1, Folasade O Oladepo1
1Vector Biology Department, Liverpool School of Tropical Medicine, Pembroke Pl, Liverpool, L3 5QA, UK.
Scientific Reports
|June 11, 2022
Summary
A new, simple method efficiently measures insecticides in long-lasting insecticide-treated nets (LLINs). This technique supports malaria prevention by enabling cost-effective monitoring of LLIN effectiveness over time.
Area of Science:
- Chemical Analysis
- Public Health
- Entomology
Background:
- Long-lasting insecticide-treated nets (LLINs) are vital for malaria prevention.
- Current methods for monitoring LLIN insecticide content are complex, expensive, and hazardous.
- Effective LLINs require sustained insecticide levels for at least three years.
Purpose of the Study:
- To develop a simple, high-throughput, low-resource method for quantifying insecticides in LLINs.
- To enable accurate tracking of insecticide levels in LLINs throughout their lifespan.
- To facilitate quality control and efficacy assessment of various LLIN formulations.
Main Methods:
- Insecticides were extracted from polyethylene-LLIN samples using a non-hazardous solvent mix and internal standard.
- Optimized High-Performance Liquid Chromatography (HPLC) enabled simultaneous detection of pyrethroid and pyriproxyfen.
- A reduced sample size (0.2 g) and solvent volume (5 ml) were utilized for efficiency.
Main Results:
- The method achieved high sensitivity, quantifying permethrin (≥0.0015%), alpha-cypermethrin (≥0.00045%), and pyriproxyfen (≥0.00025%).
- It accurately assessed various LLIN types, including Olyset® Net, Olyset® Duo, and Royal Gaurd®.
- High throughput was achieved, processing 120 samples (40 nets) simultaneously with precision and accuracy.
Conclusions:
- The developed method offers a cost-effective, rapid, and environmentally friendly alternative for LLIN insecticide monitoring.
- This innovation can significantly improve the quality control and public health impact of LLIN programs.
- Simplified monitoring will aid in ensuring the continued effectiveness of LLINs against malaria transmission.
