Related Experiment Video

Updated: Sep 20, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.7K

Myc manipulates the miRNA content and biologic functions of small cell lung cancer cell-derived small extracellular

Onur Tokgün1,2, Kubilay İnci3

  • 1Medical Genetics, Faculty of Medicine, Pamukkale University, Denizli, Turkey. onur_tokgun@hotmail.com.

Molecular Biology Reports
|June 12, 2022
PubMed
Abstract

Insights

Small extracellular vesicles (sEVs) from small cell lung cancer (SCLC) cells promote normal lung cell growth and migration. MYC gene expression influences sEV function and miRNA content, suggesting sEVs and MYC as potential SCLC therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • MYC gene amplification/overexpression occurs in 20% of small cell lung cancers (SCLCs).
  • Myc and Myc-dependent pathways are potential therapeutic targets in SCLC.
  • Small extracellular vesicles (sEVs) are implicated in cancer progression, but their association with Myc in SCLC is unexplored.

Purpose of the Study:

  • To investigate the role of Myc in regulating the function and miRNA composition of small extracellular vesicles (sEVs) in small cell lung cancer (SCLC).
  • To determine if SCLC-derived sEVs can modulate the behavior of normal lung cells.

Main Methods:

  • Small extracellular vesicles (sEVs) were isolated from SCLC cell lines (H82 and H209) with manipulated Myc expression.
  • Normal human umbilical vein endothelial cells (HUVECs) and MRC-5 lung fibroblasts were treated with SCLC-derived sEVs.
  • Cellular activity (proliferation, migration) of recipient cells was assessed using cell growth, wound healing, and transwell assays.
  • miRNA profiling of SCLC cells and their derived sEVs was performed using microarray and qRT-PCR.

Main Results:

  • SCLC-derived sEVs significantly increased proliferation and migration of normal lung cells (HUVECs and MRC-5).
  • Myc manipulation in SCLC cells altered the miRNA composition of both the cells and their released sEVs.
  • Myc was found to regulate the expression of specific miRNAs (hsa-miR-7, hsa-miR-9, hsa-miR-125b, hsa-miR-181a_2, hsa-miR-455, hsa-miR-642, hsa-miR-4417) in SCLC cells and their sEVs.

Conclusions:

  • Small extracellular vesicles (sEVs) and MYC are critical targets for SCLC therapeutic strategies.
  • MYC expression levels influence the functional properties and miRNA cargo of sEVs in SCLC.
  • SCLC-derived sEVs possess the capability to alter the behavior of normal lung cells, highlighting their role in tumor progression.
Keywords:
MYCSCLCSmall extracellular vesiclesmiRNA

More Related Videos

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool08:49

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Published on: May 27, 2016

11.3K
Author Spotlight: Exploring the Mechanisms of MicroRNA Loading into Extracellular Vesicles in Cancer Progression07:29

Author Spotlight: Exploring the Mechanisms of MicroRNA Loading into Extracellular Vesicles in Cancer Progression

Published on: October 6, 2023

1.7K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.0K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.4K