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DNA Bacteriophages

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...

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Updated: Sep 20, 2025

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Phage-mimicking nanoagents for rapid depolymerase specificity screening against multidrug resistant bacteria.

A Ringaci¹, K G Shevchenko², I V Zelepukin³

¹Moscow Institute of Physics and Technology, Dolgoprudny, Russia.

Biosensors & Bioelectronics

|June 12, 2022

Summary

This study introduces a rapid 1-minute assay for screening bacteriophages, using phage-mimicking nanoagents and surface plasmon resonance. This innovation accelerates the identification of effective phage therapies to combat antibiotic resistance.

Keywords:

Acinetobacter baumannii Gold nanoparticles Multidrug-resistant bacteria Phage screening Surface plasmon resonance

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Published on: July 21, 2023

Area of Science:

Biotechnology
Microbiology
Nanotechnology

Background:

Antibiotic resistance necessitates novel therapeutic strategies.
Bacteriophages offer a promising alternative to traditional antibiotics due to their specificity.
Current phage identification methods are time-consuming and inefficient.

Purpose of the Study:

To develop a rapid assay for bacteriophage screening.
To overcome the time limitations in matching phages to bacteria.
To facilitate the development of phage-based therapies.

Main Methods:

Utilized phage-mimicking gold nanoparticles as nanoagents.
Employed surface plasmon resonance for detection.
Assessed nanoagent specificity and enzymatic activity in real-time.

Main Results:

Developed a 1-minute bacteriophage screening assay.
Demonstrated real-time measurement of phage specificity and enzymatic activity.
Enabled direct assessment of nanoagent convergence on bacterial cell walls.
Detected nanoagent detachment indicating cell capsule destruction.

Conclusions:

The novel assay significantly reduces phage-bacteria matching time.
This method can accelerate the adoption of phage therapy.
Phage-mimicking nanoagents provide a powerful tool for evaluating phage efficacy.