Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Patch Clamp01:18

Patch Clamp

5.7K
Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Upregulation of alveolar fluid clearance is not sufficient for Na<sup>+</sup>,K<sup>+</sup>-ATPase β subunit-mediated gene therapy of LPS-induced acute lung injury in mice.

Scientific reports·2026
Same author

Anticodon-edited transfer RNAs (ACE-tRNAs) encoded as therapeutic nonviral minimal DNA vectors.

Nucleic acids research·2026
Same author

Biomolecular Condensates Can Induce Local Membrane Potentials.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Subnuclear organization and mislocalization of plasmids reduce transgene expression.

Molecular therapy. Nucleic acids·2025
Same author

Anticodon Edited Transfer RNAs (ACE-tRNAs) Encoded as Therapeutic Nonviral Minimal DNA Vectors.

bioRxiv : the preprint server for biology·2025
Same author

Electroporation- and Liposome-Mediated Co-Transfection of Single and Multiple Plasmids.

Pharmaceutics·2025

Related Experiment Video

Updated: Sep 8, 2025

Author Spotlight: Optimizing Porous Substrate Electroporation Through Micro and Nanochannels for Enhanced Monitoring and Intermediate Stage Characterization
08:06

Author Spotlight: Optimizing Porous Substrate Electroporation Through Micro and Nanochannels for Enhanced Monitoring and Intermediate Stage Characterization

Published on: September 27, 2024

635

Modeling and simulation of current-clamp electroporation.

Anthony Gurunian1, David A Dean1

  • 1Department of Pediatrics, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA.

Bioelectrochemistry (Amsterdam, Netherlands)
|June 12, 2022
PubMed
Summary

Current-clamp electroporation simulations reveal voltage fluctuations may arise from multiple pores, not just one. The Langevin model offers greater accuracy than the Smoluchowski model for few-pore scenarios.

Keywords:
ChronopotentiometryElectroporationHopf BifurcationLangevinSmoluchowski

More Related Videos

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds
08:23

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds

Published on: August 19, 2025

59
The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
10:34

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

2.9K

Related Experiment Videos

Last Updated: Sep 8, 2025

Author Spotlight: Optimizing Porous Substrate Electroporation Through Micro and Nanochannels for Enhanced Monitoring and Intermediate Stage Characterization
08:06

Author Spotlight: Optimizing Porous Substrate Electroporation Through Micro and Nanochannels for Enhanced Monitoring and Intermediate Stage Characterization

Published on: September 27, 2024

635
High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds
08:23

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds

Published on: August 19, 2025

59
The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
10:34

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

2.9K

Area of Science:

  • Biophysics
  • Membrane Electroporation

Background:

  • Current-clamp electroporation involves applying constant current across membranes, causing voltage fluctuations due to electropore formation.
  • This technique enables long-timescale electroporation measurements and comparisons with theoretical models.
  • A key question is whether current-clamp electroporation exclusively creates a single pore.

Purpose of the Study:

  • To investigate the mechanisms behind voltage fluctuations during current-clamp electroporation.
  • To determine if current-clamp electroporation can result in the formation of more than one pore.
  • To compare the accuracy of the Smoluchowski and Langevin models in simulating electroporation.

Main Methods:

  • Simulated current-clamp electroporation using Smoluchowski and Langevin equations.
  • Analyzed voltage fluctuations to identify pore formation mechanisms.

Main Results:

  • Identified two potential mechanisms for voltage fluctuations: a single pore with negative feedback or a population of pores opening/closing.
  • Demonstrated that current-clamp conditions do not necessitate the creation of a single pore.
  • Found the Langevin model to be more accurate than the Smoluchowski model when few pores are present.

Conclusions:

  • Current-clamp electroporation can involve multiple pores, challenging the single-pore hypothesis.
  • The choice of simulation model (Langevin vs. Smoluchowski) impacts accuracy, particularly in low-pore regimes.
  • These findings refine our understanding of electroporation dynamics and modeling.