Acceleration of lactate-utilizing pathway for enhancing biohydrogen production by magnetite supplementation in
Do-Hyung Kim1, Jeong-Jun Yoon2, Sang-Hyoun Kim3
1Green and Sustainable Materials R&D Department, Korea Institute of Industrial Technology (KITECH), Cheonan-si 31056, Republic of Korea; School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea.
Abstract:
A conductive metal compound can be used as a catalyst for enhancing hydrogen production by dark fermentation. This study aimed to identify mechanisms of enhanced hydrogen production by magnetite supplementation. Experiments were performed with lactate and/or magnetite supplementation to confirm that the lactate-utilizing pathway is the key cause of enhanced hydrogen production. Also, ribonucleic acid sample was collected for monitoring gene regulation under each condition. Hydrogen production was significantly enhanced by approximately 25.6% and 58.9%, respectively, via magnetite alone and with lactate. Moreover, the expression of genes involved in hydrogen production, including pyruvate ferredoxin oxidoreductase, hydrogenase, and ferredoxin, via magnetite alone and with lactate was upregulated by 0.26, 0.71, and 3.50 and 1.06, 2.14, and 1.94 times, respectively.
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