Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of hypergravity on the biomechanics of the musculoskeletal system in human lumbar spine: a numerical study.

Frontiers in bioengineering and biotechnology·2026
Same author

H<sub>2</sub>O<sub>2</sub>-independent oxygen activation via proton-coupled electron transfer for selective hydroxyl radical generation.

Water research·2026
Same author

Recyclable glass fiber-reinforced epoxy copper clad laminates for printed circuit board.

Communications chemistry·2026
Same author

Associations of Mycoplasma pneumoniae load, co-infections, and macrolide resistance with clinical-laboratory profiles in hospitalized pediatric pneumonia: a targeted next-generation sequencing study of bronchoalveolar lavage fluid.

Annals of clinical microbiology and antimicrobials·2026
Same author

Comparative analysis of lipid-lowering mechanisms in efficacy-divergent traditional Chinese medicines.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

Sensitivity of various material properties to the intervertebral disc biomechanics.

Computer methods in biomechanics and biomedical engineering·2026

Related Experiment Video

Updated: Sep 8, 2025

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
10:06

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Published on: October 19, 2013

22.4K

Expression analysis of nel during zebrafish embryonic development.

Jinxiang Zhao1, Guanyun Wei2, Jiang Zhu1

  • 1The Affiliated Suqian First People's Hospital of Nanjing Medical University, Suqian, China.

Gene Expression Patterns : GEP
|June 12, 2022
PubMed
Summary

Nel family genes are crucial for zebrafish neural development. Nell2a and Nell2b are conserved and expressed in the nervous system and eyes, while Nell3 shows distinct expression patterns.

Keywords:
DevelopmentExpressionNelZebrafish

More Related Videos

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
10:00

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

Published on: January 12, 2015

14.7K
Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
12:31

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

Published on: March 25, 2009

23.4K

Related Experiment Videos

Last Updated: Sep 8, 2025

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
10:06

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Published on: October 19, 2013

22.4K
Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
10:00

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

Published on: January 12, 2015

14.7K
Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
12:31

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

Published on: March 25, 2009

23.4K

Area of Science:

  • Developmental Biology
  • Neuroscience
  • Genetics

Background:

  • Nel is a multimeric extracellular glycoprotein vital for neural development and function.
  • Three nel paralogues (nell2a, nell2b, nell3) exist in zebrafish, but their expression is not fully understood.

Purpose of the Study:

  • To conduct a phylogenetic analysis of the nel family across seven species.
  • To determine the expression profiles of nell2a, nell2b, and nell3 in zebrafish embryos.

Main Methods:

  • Phylogenetic analysis of nel family genes.
  • In situ hybridization to detect gene expression in zebrafish embryos.

Main Results:

  • Nell2a and Nell2b are highly conserved across species.
  • Nel genes are enriched in the zebrafish central nervous system, with specific regional distributions.
  • Nell2a and Nell2b show expression in the nervous system and eyes, while Nell3 has a unique expression pattern in the brain and spinal cord.

Conclusions:

  • Nell2a and Nell2b are broadly expressed in the developing zebrafish nervous system and eyes.
  • Nell3 exhibits a distinct expression profile, differing significantly from nell2a and nell2b.
  • This study enhances understanding of the nel gene family's role in zebrafish embryonic development.