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Quantifying up to 90 polyphenols simultaneously in human bio-fluids by LC-MS/MS.

Ian Oesterle1, Dominik Braun2, Annette Rompel3

  • 1Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, 1090, Vienna, Austria; Doctoral School of Chemistry, University of Vienna, 1090, Vienna, Austria; Universität Wien, Fakultät für Chemie, Institut für Biophysikalische Chemie, 1090, Wien, Österreich.

Analytica Chimica Acta
|June 12, 2022
PubMed
Summary

A new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method quantifies 90 polyphenols in human samples. This cost-effective, high-throughput method enables biomonitoring for health studies.

Keywords:
ExposomeHuman biomonitoring (HBM)PlasmaSerumUrine

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Area of Science:

  • Biomarkers and Metabolomics
  • Analytical Chemistry
  • Nutritional Science

Background:

  • Human biomonitoring (HBM) of polyphenols is crucial for understanding their health effects, such as antioxidant and anti-inflammatory properties.
  • Existing methods may not be suitable for large-scale studies due to sample volume, cost, or time constraints.

Purpose of the Study:

  • To develop and validate a targeted LC-MS/MS method for quantifying a wide range of polyphenols in human urine, serum, and plasma.
  • To establish a cost- and time-efficient sample preparation protocol for high-throughput analysis applicable to large cohort and exposome-wide association studies.

Main Methods:

  • Development of a targeted LC-MS/MS method capable of quantifying up to 90 analytes across major polyphenol classes.
  • Optimization of a sample preparation protocol for low sample volumes, achieving high extraction efficiencies (98% for urine, 98% for serum, 87% for plasma).
  • In-house validation of the method against stringent requirements, assessing performance across various concentration levels.

Main Results:

  • The method demonstrated low limits of detection (0.11–340 ng/mL) across sample types.
  • A significant number of analytes met validation requirements: 66 in urine, 49 in serum, and 64 in plasma.
  • A proof-of-principle study successfully detected 39 polyphenols in urine post-ingestion of a polyphenol-rich smoothie, with phase II metabolites being more abundant.

Conclusions:

  • The developed LC-MS/MS method is suitable for high-throughput human biomonitoring of polyphenols in urine, serum, and plasma.
  • The method's efficiency and validation performance support its application in large-scale epidemiological and exposome studies.
  • The study highlights the utility of biomonitoring for assessing polyphenol exposure and metabolic profiles in humans.