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Tannic acid attenuate AKT phosphorylation to inhibit UMUC3 bladder cancer cell proliferation

Ming-Cheng Chen1,2, Selvaraj Annseles Rajula3, V Bharath Kumar4

  • 1Division of Colorectal Surgery, Department of Surgery, Taichung Veterans General Hospital, Taichung, Taiwan.

Molecular and Cellular Biochemistry
|June 12, 2022
PubMed

Insights

Tannic acid (TA) effectively inhibits human bladder cancer cell growth and survival. This natural compound reduces cancer cell viability and promotes apoptosis, offering potential as a novel bladder cancer treatment.

Area of Science:

  • Oncology
  • Biochemistry

Background:

  • Urothelial bladder cancer incidence is rising in Western countries.
  • Current therapies offer limited efficacy, necessitating new treatment strategies.

Purpose of the Study:

  • To evaluate the anticancer potential of Tannic acid (TA) against human bladder cancer cells.
  • To elucidate the molecular mechanisms underlying TA's effects on bladder cancer.

Main Methods:

  • Human bladder cancer cells (UMUC3) were treated with varying concentrations of Tannic acid (TA).
  • Assays included cell viability, colony formation, apoptosis, Western blotting for protein expression, and analysis of the PI3K/Akt pathway.

Main Results:

  • TA significantly inhibited bladder cancer cell viability and colony formation.
  • TA induced apoptosis by decreasing anti-apoptotic proteins (BCL2, MCL-1, BCL-XL) and activating Caspase-3.
  • TA reduced expression of stem cell markers (SOX2, OCT4, NANOG) and phosphorylation of Akt.

Conclusions:

  • Tannic acid exhibits significant growth inhibitory effects on human bladder cancer cells.
  • TA demonstrates potential as a therapeutic agent for bladder cancer, possibly through modulation of the PI3K/Akt pathway.
Keywords:
ApoptosisBCL-XLl UMUC3BCL2Bladder cancerCaspaseMCL-1NANOGSOX2Tannic acid

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