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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Sep 8, 2025

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Published on: March 20, 2018

A Comprehensive Database for DNA Adductomics.

Giorgia La Barbera¹, Katrine Dalmo Nommesen¹, Catalina Cuparencu¹

¹Department of Nutrition Exercise and Sports, University of Copenhagen, Frederiksberg, Denmark.

Frontiers in Chemistry

|June 13, 2022

Summary

This study introduces a comprehensive DNA adducts database to improve the identification of DNA modifications. The database aids researchers in understanding carcinogenesis by providing a faster, more efficient tool for analyzing DNA adducts.

Keywords:

DNA adduct carcinogenesis database identification mass spectrometry toxicology

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Last Updated: Sep 8, 2025

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

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Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

Area of Science:

Environmental Chemistry
Toxicology
Bioinformatics

Background:

Genotoxic compounds can cause DNA adducts, potentially leading to cancer.
DNA adductomics, using liquid chromatography-high-resolution mass spectrometry (LC-HRMS), is emerging for adduct screening.
Current limitations in databases and bioinformatics tools hinder DNA adductomics applicability.

Purpose of the Study:

To develop and curate a comprehensive database for annotating DNA adducts in biological samples.
To enhance the efficiency and accuracy of DNA adduct identification in LC-HRMS analyses.
To provide insights into DNA modifications resulting from various genotoxicants.

Main Methods:

A four-step approach was used: literature review, structural harmonization, database expansion with new adducts, and spectral library construction.
Included literature-curated adducts identified by reference standards, NMR, or HRMS/MS.
Generated in silico MS/MS fragments and acquired experimental spectra for 15 reference standards.

Main Results:

A systematic database of 279 DNA adducts was compiled, harmonizing structures, formulas, and names.
The database was expanded to 582 entries, including 303 potential adducts with detailed chemical and structural information.
A preliminary spectral library with experimental and predicted MS/MS data was created.

Conclusions:

The publicly available DNA adducts database (https://gitlab.com/nexs-metabolomics/projects/dna_adductomics_database) is a valuable tool for (HR)MS data annotation.
Inclusion of metadata enhances prioritization and confidence in DNA adduct identification.
Future integration with authentic MS/MS spectra and user-friendly bioinformatics tools will further improve adduct identification.