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Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues.

Yongjun Jang1, Da Jung Jung2, Seung-Cheol Choi3

  • 1Department of Biomedical Sciences, College of Medicine, Korea University Seoul Korea envokim72@korea.ac.kr ydpark67@korea.ac.kr.

RSC Advances
|June 13, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed a simple method to create multi-dimensional cardiac macro tissues (mCMTs) using layer-by-layer cell deposition. These engineered tissues exhibit synchronized beating and enhanced maturation, offering a promising platform for drug testing.

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Constructing multi-dimensional cardiac tissue is challenging due to difficulties in cardiomyocyte maturation and synchronization with other cardiac cell types.
  • Current biofabrication methods struggle to replicate the complex cellular architecture and function of native cardiac tissue.

Purpose of the Study:

  • To develop a simple and efficient method for fabricating multi-dimensional cardiac macro tissue (mCMT) with synchronized beating.
  • To enhance the maturation and functional characteristics of engineered cardiac tissue for in vitro applications.

Main Methods:

  • Utilized layer-by-layer (LBL) deposition of cells onto micro-patterned polydimethylsiloxane (PDMS) substrates.
  • Employed pluripotent stem cell (PSC)-derived cardiomyocytes and cardiac fibroblasts for tissue construction.
  • Incorporated cardiac maturation factors and growth factors during the fabrication and maturation process.

Main Results:

  • Successfully fabricated mCMTs exhibiting synchronized beating characteristics.
  • Demonstrated significantly enhanced gene expression of cardiac maturation markers (MLC2v, cTNI) and improved sarcomere formation.
  • mCMTs showed improved tissue-like structures and functions compared to conventional methods.

Conclusions:

  • The LBL deposition method provides an effective strategy for creating mature, functional cardiac tissue constructs.
  • Fabricated mCMTs serve as a valuable in vitro platform for drug efficacy and toxicity screening.
  • This approach has broad implications for diverse in vitro studies requiring physiologically relevant tissue models.