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Growth-stimulatory action of plasma membrane-associated growth factor. A synergistic effect with platelet-poor plasma

T Nagasaki, M A Lieberman

    Experimental Cell Research
    |May 1, 1987
    PubMed

    Insights

    Mouse liver plasma membrane-associated growth factor (PMGF) requires a plasma cofactor for fibroblast proliferation. This growth factor acts as a competence factor, distinct from progression factors.

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Biochemistry

    Background:

    • Growth factors are crucial for cell proliferation.
    • Plasma membrane-associated growth factors (PMGF) play a role in cellular regulation.
    • Understanding the specific roles of different growth factors is essential for cell biology research.

    Purpose of the Study:

    • To investigate the growth-stimulatory action of partially purified PMGF on cultured mouse fibroblasts.
    • To determine the conditions under which PMGF supports fibroblast proliferation.
    • To characterize PMGF's mechanism of action and compare it to known growth factors.

    Main Methods:

    • Partial purification of growth factor activity from mouse liver plasma membranes.
    • Culturing mouse fibroblasts and assessing proliferation.
    • Stimulating DNA synthesis in quiescent fibroblasts.
    • Comparing PMGF activity with platelet-derived growth factor (PDGF) and fibroblast growth factor (FGF).

    Main Results:

    • PMGF alone did not support fibroblast proliferation in monolayer cultures.
    • PMGF stimulated fibroblast growth synergistically with human platelet-poor plasma (h-CMP).
    • PMGF and h-CMP together stimulated DNA synthesis in quiescent fibroblasts.
    • PMGF's synergistic action with h-CMP was partially mimicked by insulin and insulin-like growth factor I (IGF-I).

    Conclusions:

    • PMGF functions as a competence factor, requiring a progression factor (like those in h-CMP) for full mitogenic activity.
    • PMGF's mechanism of action is distinct from progression factors.
    • The findings contribute to understanding the complex regulation of cell proliferation by growth factors.

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