Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrophilic Aromatic Substitution: Sulfonation of Benzene01:22

Electrophilic Aromatic Substitution: Sulfonation of Benzene

6.4K
Sulfonation of benzene is a reaction wherein benzene is treated with fuming sulfuric acid at room temperature to produce benzenesulfonic acid. Fuming sulfuric acid is a mixture of sulfur trioxide and concentrated sulfuric acid.
6.4K
Protein and Protein Structure02:15

Protein and Protein Structure

81.1K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
81.1K
Protein Folding01:22

Protein Folding

120.7K
Overview
120.7K
Regioselectivity and Stereochemistry of Acid-Catalyzed Hydration02:34

Regioselectivity and Stereochemistry of Acid-Catalyzed Hydration

8.6K
The rate of acid-catalyzed hydration of alkenes depends on the alkene's structure, as the presence of alkyl substituents at the double bond can significantly influence the rate.
8.6K
Protein Organization01:24

Protein Organization

7.0K
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence....
7.0K
Reactions at the Benzylic Position: Halogenation01:11

Reactions at the Benzylic Position: Halogenation

2.7K
Benzylic halogenation takes place under conditions that favor radical reactions such as heat, light, or a free radical initiator like peroxide.
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

β-Sheet Polyelectrolyte Nanostructures: Design, Self-Assembly, In Vitro, and In Vivo Biological Activity.

Advanced healthcare materials·2026
Same author

Autodegradable Polyzwitterionic Nanoplatform to Target and Suppress Solid Tumor in Mice Xenografts.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Versatile Polypeptide-Anchored Antifouling Dip-Coatings for Common Medical Implant Materials.

ACS applied materials & interfaces·2025
Same author

Unimolecular Micelle for MLN8237 Delivery to Target AURKA-RalA Crosstalk for Ras-Driven Tumor Suppression in Mice Xenografts.

Biomacromolecules·2025
Same author

Tweaking Unimolecular Micellar Nanoarchitecture for Drug Delivery in Tumor Xenograft Mice Models.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

ROPISA Strategy for In-Situ Loading in Polypeptide Nanoparticles.

Chemistry, an Asian journal·2025

Related Experiment Video

Updated: Sep 8, 2025

Synthesis and Characterization of 1,2-Dithiolane Modified Self-Assembling Peptides
09:54

Synthesis and Characterization of 1,2-Dithiolane Modified Self-Assembling Peptides

Published on: August 20, 2018

7.3K

Tertiary-Butylbenzene Functionalization as a Strategy for β-Sheet Polypeptides.

Rahul Nisal1, Manickam Jayakannan1

  • 1Department of Chemistry, Indian Institute of Science Education and Research (IISER Pune), Dr. Homi Bhabha Road, Pune 411008, Maharashtra, India.

Biomacromolecules
|June 13, 2022
PubMed
Summary

Researchers developed a new method to synthesize soluble β-sheet polypeptides using t-butylbenzene functionalization. This steric-hindrance approach overcomes uncontrolled precipitation in ring-opening polymerization (ROP), enabling controlled synthesis of high-molecular-weight polypeptides.

More Related Videos

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
11:09

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation

Published on: August 1, 2018

10.8K
Efficient Synthesis of Polyfunctionalized Benzenes in Water via Persulfate-promoted Benzannulation of α,β-Unsaturated Compounds and Alkynes
05:34

Efficient Synthesis of Polyfunctionalized Benzenes in Water via Persulfate-promoted Benzannulation of α,β-Unsaturated Compounds and Alkynes

Published on: December 16, 2019

8.0K

Related Experiment Videos

Last Updated: Sep 8, 2025

Synthesis and Characterization of 1,2-Dithiolane Modified Self-Assembling Peptides
09:54

Synthesis and Characterization of 1,2-Dithiolane Modified Self-Assembling Peptides

Published on: August 20, 2018

7.3K
Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
11:09

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation

Published on: August 1, 2018

10.8K
Efficient Synthesis of Polyfunctionalized Benzenes in Water via Persulfate-promoted Benzannulation of α,β-Unsaturated Compounds and Alkynes
05:34

Efficient Synthesis of Polyfunctionalized Benzenes in Water via Persulfate-promoted Benzannulation of α,β-Unsaturated Compounds and Alkynes

Published on: December 16, 2019

8.0K

Area of Science:

  • Polymer Chemistry
  • Organic Synthesis
  • Biomaterials Science

Background:

  • β-Sheet forming polypeptides are challenging to synthesize due to uncontrolled precipitation during ring-opening polymerization (ROP).
  • Existing synthetic methods lack control over chain growth and solubility for these important biomaterials.

Purpose of the Study:

  • To introduce a novel t-butylbenzene functionalization strategy to enable controlled synthesis of soluble β-sheet polypeptides.
  • To overcome precipitation issues in ROP and achieve high molecular weights with narrow dispersity.

Main Methods:

  • Design and synthesis of bulky N-carboxyanhydride monomers with t-butylbenzene pendants.
  • Utilizing N-heterocyclic carbene catalysis for ring-opening polymerization.
  • Employing real-time Fourier transform infrared and 1H NMR spectroscopy to study ROP kinetics and secondary structures.

Main Results:

  • Achieved homogeneous polymerization with controlled chain growth for poly(l-serine) and poly(l-cysteine).
  • Synthesized soluble polypeptides in high molecular weights (Mn = 32 kDa) with narrow dispersity (Đ ≤ 1.3).
  • Demonstrated that α-helical conformational fronts in propagating chains accelerate ROP kinetics.

Conclusions:

  • The t-butylbenzene-substituted steric-hindrance approach successfully yields soluble, high-molecular-weight polypeptides.
  • This method provides a valuable platform for exploring new polypeptide architectures and applications.
  • Reversible conformational transitions can restore β-sheet structures post-deprotection, offering further control over material properties.