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Long Non-Coding RNA DUXAP8 Acts as an Oncogene in Sinonasal Squamous Cell Carcinoma Through miR-584-5p/FNDC3B Pathway

Xuan Chen1, Guidi Li1, Guanzhong Zhong2

  • 1Department of Otolaryngology, Head and Neck Surgery, 477688Shunde Hospital of Southern Medical University, Foshan, Guangdong Province, China.

American Journal of Rhinology & Allergy
|June 13, 2022
PubMed

Insights

Long non-coding RNA DUXAP8 acts as an oncogene in sinonasal squamous cell carcinoma (SNSCC). Silencing DUXAP8 suppresses SNSCC progression by regulating FNDC3B expression, suggesting DUXAP8 as a potential molecular marker for SNSCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Sinonasal squamous cell carcinoma (SNSCC) is a rare malignancy of the head and neck.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.
  • The specific function of lncRNA DUXAP8 in SNSCC remains largely unexplored.

Purpose of the Study:

  • To investigate the role and underlying mechanism of lncRNA DUXAP8 in the progression of SNSCC.
  • To determine the relationship between DUXAP8, microRNA-584-5p (miR-584-5p), and fibronectin type III domain containing 3B (FNDC3B) in SNSCC.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) for gene expression analysis.
  • In vitro assays including proliferation (colony formation, CCK-8, EdU), migration, invasion, and apoptosis (TUNEL, JC-1).
  • Mechanism studies involving RNA pull-down, RNA immunoprecipitation (RIP), and luciferase reporter assays.

Main Results:

  • DUXAP8 was found to be overexpressed in SNSCC cells.
  • Silencing DUXAP8 significantly inhibited SNSCC cell proliferation, migration, and invasion, while promoting apoptosis.
  • DUXAP8 was identified to up-regulate FNDC3B expression by sponging miR-584-5p.
  • Rescue experiments confirmed that DUXAP8 promotes SNSCC progression via FNDC3B upregulation.

Conclusions:

  • DUXAP8 functions as an oncogene in SNSCC.
  • The DUXAP8/miR-584-5p/FNDC3B axis plays a critical role in SNSCC pathogenesis.
  • DUXAP8 represents a potential novel molecular marker and therapeutic target for SNSCC.
Keywords:
DUXAP8FNDC3BmiR-584-5psinonasal squamous cell carcinoma

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