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LncRNA DSCAM-AS1 Negatively Interacts with miR-124 to Promote Hepatocellular Carcinoma Proliferation

Zheng Wang1, Shengmian Li2, Gaili Zhang3

  • 1Department of Gastroenterology, Hebei Medical University, Shijiazhuang City, Hebei Province, 050000, P.R. China.

Critical Reviews in Eukaryotic Gene Expression
|June 13, 2022
PubMed

Insights

Long non-coding RNA DSCAM antisense RNA 1 (DSCAM-AS1) promotes hepatocellular carcinoma (HCC) proliferation by interacting with miR-124. This study reveals DSCAM-AS1 as a potential therapeutic target for HCC, as its upregulation correlates with disease progression.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNA DSCAM antisense RNA 1 (DSCAM-AS1) is implicated in chemotherapy resistance in breast cancer.
  • Understanding the role of DSCAM-AS1 in other cancers, like hepatocellular carcinoma (HCC), is crucial.

Purpose of the Study:

  • To investigate the differential expression and functional role of DSCAM-AS1 and miR-124 in HCC.
  • To explore the interaction between DSCAM-AS1 and miR-124 in HCC progression.

Main Methods:

  • Quantitative polymerase chain reaction (qPCR) for gene expression analysis.
  • Paired t-test and ANOVA Tukey test for statistical comparisons.
  • CCK-8 assay to assess cell proliferation after transfections.

Main Results:

  • DSCAM-AS1 was upregulated and miR-124 was downregulated in HCC tissues compared to non-tumor tissues.
  • DSCAM-AS1 and miR-124 showed an inverse correlation in HCC tissues, with levels changing with clinical stage.
  • DSCAM-AS1 overexpression promoted HCC cell proliferation, while miR-124 overexpression inhibited it. DSCAM-AS1 counteracted miR-124's effects.

Conclusions:

  • DSCAM-AS1 promotes HCC proliferation and is inversely correlated with miR-124.
  • The DSCAM-AS1/miR-124 interaction plays a significant role in HCC development and progression.
  • DSCAM-AS1 represents a potential therapeutic target for hepatocellular carcinoma.

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