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Updated: Sep 8, 2025

Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions
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A close shave: How SARS-CoV-2 induces the loss of cilia

Barbara F Fonseca1,2, Lisa A Chakrabarti1

  • 1Control of Chronic Viral Infections Group, Virus and Immunity Unit, Institut Pasteur, Université Paris Cité, CNRS UMR 3569, Paris, France.

The Journal of Cell Biology
|June 13, 2022
PubMed

Insights

The SARS-CoV-2 ORF10 protein boosts E3 ligase CUL2ZYG11B activity, degrading ciliary proteins. This loss of cilia may aid virus spread in the respiratory tract.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • SARS-CoV-2 infection impacts respiratory cells.
  • Cilia play crucial roles in respiratory tract clearance.
  • Viral proteins can manipulate host cell machinery.

Purpose of the Study:

  • To investigate the function of SARS-CoV-2 protein ORF10.
  • To understand how ORF10 affects host cell components.
  • To determine the role of ORF10 in viral pathogenesis.

Main Methods:

  • Investigated protein-protein interactions.
  • Assessed E3 ligase activity.
  • Analyzed ciliary protein levels via Western blotting and immunofluorescence.

Main Results:

  • SARS-CoV-2 ORF10 protein enhances CUL2ZYG11B E3 ligase activity.
  • ORF10 induces degradation of multiple ciliary proteins.
  • Loss of cilia observed in infected cells.

Conclusions:

  • SARS-CoV-2 ORF10 targets ciliary proteins for degradation.
  • Cilia dysfunction may promote SARS-CoV-2 spread.
  • ORF10-mediated ciliary disruption is a potential viral mechanism.

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