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OsCPK29 interacts with MADS68 to regulate pollen development in rice.

Rajeev Ranjan1, Naveen Malik2, Shivam Sharma3

  • 1National Institute of Plant Genome Research (NIPGR), New Delhi 110067, India; Interdisciplinary Centre for Plant Genomics and Department of Plant Molecular Biology, University of Delhi, South Campus (UDSC), New Delhi 110021, India.

Plant Science : an International Journal of Experimental Plant Biology
|June 13, 2022
PubMed
Summary

Rice Calcium-dependent protein kinase 29 (OsCPK29) is crucial for pollen development. Its absence leads to non-viable pollen, reduced fertility, and fewer seeds, highlighting its role in plant reproduction.

Keywords:
Calcium-dependent protein kinaseIntineMADS-box transcription factorNuclear localized CPKPollen developmentRice

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Area of Science:

  • Plant Biology
  • Molecular Genetics
  • Reproductive Biology

Background:

  • Calcium-dependent protein kinases (CPKs) are vital signaling regulators in plant growth.
  • Successful reproduction in flowering plants depends on proper pollen development and germination.

Purpose of the Study:

  • To functionally characterize a novel rice CPK, OsCPK29, and elucidate its role in pollen development.
  • To investigate the molecular mechanisms underlying OsCPK29's function in rice anthers.

Main Methods:

  • Gene expression analysis and subcellular localization of OsCPK29.
  • Generation and analysis of OsCPK29 knockdown rice plants.
  • Cytological and microscopic examination of anther and pollen development.
  • In vitro and in vivo interaction studies with MADS68.

Main Results:

  • OsCPK29 is primarily expressed in maturing rice anthers and localizes to the nucleus.
  • OsCPK29 knockdown resulted in male sterility, characterized by non-viable pollen lacking a functional intine layer.
  • Downregulation of intine development genes and interaction with MADS68 were observed in OsCPK29-silenced anthers.

Conclusions:

  • OsCPK29 plays a critical role in rice pollen development, specifically in intine formation.
  • OsCPK29 acts as a key regulator, potentially through interaction with MADS68, ensuring male fertility in rice.