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Updated: Sep 8, 2025

Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism Microcystis aeruginosa Using Flow Cytometry
Published on: January 29, 2016
High-throughput characterization of the filamentous cyanobacterium Anabaena sp. using flow cytometry
Philipp Doppler1, Ricarda Kriechbaum1, Oliver Spadiut1
1Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria.
Abstract:
In stirred-tank photobioreactors agitation causes fragmentation of filamentous cyanobacteria. Here, we introduce a flow cytometric approach for high-throughput measurements of trichome dimensions, heterocysts and metabolic activity of Anabaena sp. cultures. The longest characterized trichome had 1135 μm chain length. This technology could potentially be used for monitoring and control purposes.

