A simple method for the measurement of blood cholinesterase activities under field conditions

A new separation method using a hand-driven discoidal micro-centrifuge was developed. It is suitable for separating red cells from plasma in field conditions. The amounts of plasma produced are sufficient to be used for the dip-stick estimation of pseudocholinesterase activity. The strip estimations of plasma pseudochoninesterase activity correlated well with the spectrophotometric method of plasma cholinesterase. The test paper methods seem to be reliable under field conditions in acute organophosphate poisonings. To verify the results of the paper strip methods the blood method absorbed on paper can be used. This method is applicable for the measuring of blood cholinesterase activities and thus monitoring humans exposed to organophosphates under field conditions.