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Related Experiment Video

Updated: Sep 8, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

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Microfluidic cell sorter sample preparation for genomic assays.

Nicole Jagnandan1, Jose Morachis1

  • 1Applications, NanoCellect Biomedical Inc., San Diego, California 92121, USA.

Biomicrofluidics
|June 14, 2022
PubMed
Summary

High-quality single-cell RNA sequencing (scRNA-seq) requires effective cell preparation. Microfluidic cell sorting offers a gentle, high-purity method for removing debris and unwanted cells, improving genomic data quality.

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Area of Science:

  • Genomics
  • Cell Biology
  • Biotechnology

Background:

  • Single-cell RNA sequencing (scRNA-seq) enables discovery of rare cell populations and mutations.
  • Sample preparation quality is crucial for scRNA-seq, impacting RNA integrity.
  • Traditional cell sorting methods like magnetic beads are limited, while droplet sorters can be complex and stressful for cells.

Purpose of the Study:

  • To evaluate microfluidic cell sorting for sample preparation in scRNA-seq.
  • To demonstrate the effectiveness of gentle sorting technology in improving cell purity and RNA quality.
  • To assess the impact of microfluidic sorting on genomic data yield and library complexity.

Main Methods:

  • Utilized microfluidic-based cell sorters (WOLF® and WOLF G2®) for cell enrichment.

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  • Employed gentle sorting technology to remove debris and unwanted cells.
  • Integrated microfluidic sorting with cDNA generation and library preparation for scRNA-seq.

    • Main Results:

    • Microfluidic sorting successfully removed debris and unwanted cells, enhancing sample purity.
    • The gentle sorting process preserved RNA quality.
    • Resulting genomic libraries showed improved complexity and higher data yield per cell.

    Conclusions:

    • Microfluidic cell sorting is an effective technique for scRNA-seq sample preparation.
    • Gentle microfluidic sorting improves cell purity, RNA quality, and downstream genomic data.
    • This technology enhances the reliability and efficiency of single-cell transcriptomic studies.