Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

6.1K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
6.1K
Next-generation Sequencing03:00

Next-generation Sequencing

92.5K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
92.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Brief Case: Adjudication of discrepant genotypic and phenotypic antimicrobial susceptibility testing results in a patient with <i>Staphylococcus aureus</i> bacteremia.

Journal of clinical microbiology·2026
Same author

A spatial transcriptomics dataset of pancreas sections in normal glucose tolerance and type 2 diabetic donors.

Scientific data·2025
Same author

Potentially prebiotic isocyanide activation chemistry drives RNA assembly.

Chemical communications (Cambridge, England)·2025
Same author

Potentially Prebiotic Isocyanide Activation Chemistry Drives RNA Assembly via both Nonenzymatic and Ribozymecatalyzed Ligation.

bioRxiv : the preprint server for biology·2025
Same author

RNA Mango-based sensors for lead.

Organic & biomolecular chemistry·2025
Same author

Evolution of the substrate specificity of an RNA ligase ribozyme from phosphorimidazole to triphosphate activation.

Proceedings of the National Academy of Sciences of the United States of America·2024

Related Experiment Video

Updated: Sep 8, 2025

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

34.0K

REVERSE: a user-friendly web server for analyzing next-generation sequencing data from in vitro selection/evolution

Zoe Weiss1, Saurja DasGupta2,3,4

  • 1Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.

Nucleic Acids Research
|June 14, 2022
PubMed
Summary
This summary is machine-generated.

REVERSE is a new web server that simplifies next-generation sequencing data analysis for RNA selection experiments. It provides rapid, user-friendly tools for researchers without extensive programming skills.

More Related Videos

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
09:14

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

Published on: June 28, 2018

7.2K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

11.9K

Related Experiment Videos

Last Updated: Sep 8, 2025

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

34.0K
Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
09:14

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

Published on: June 28, 2018

7.2K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

11.9K

Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Computational Biology

Background:

  • Next-generation sequencing (NGS) is crucial for analyzing in vitro selection/evolution experiments, offering single-nucleotide resolution of nucleic acid sequences.
  • Analyzing large NGS datasets can be challenging for researchers lacking advanced computational expertise.
  • In vitro selection and evolution are powerful techniques for discovering functional nucleic acid sequences.

Purpose of the Study:

  • To develop a user-friendly web server, REVERSE (Rapid EValuation of Experimental RNA Selection/Evolution), for streamlined analysis of NGS data from RNA selection experiments.
  • To provide an integrated computational pipeline that performs pre-processing and detailed sequence-level analyses rapidly.
  • To make complex NGS data analysis accessible to researchers with limited programming skills.

Main Methods:

  • Developed REVERSE, a web server with a graphical user interface implementing an integrated computational pipeline.
  • Implemented quality filtering, dereplication, and trimming of raw FASTQ files.
  • Designed two analysis pipelines: one for sequence and motif enrichment tracking, and another for sequence clustering and conservation analysis.

Main Results:

  • REVERSE processes raw FASTQ files, performing quality control and preparing data for analysis within minutes.
  • Pipeline 1 quantifies unique sequences, tracks motif enrichment, and identifies mutational intermediates.
  • Pipeline 2 clusters similar sequences, monitors peak sequence enrichment, and generates consensus sequences with nucleotide conservation analysis.
  • Both pipelines produce downloadable data spreadsheets and high-resolution figures.

Conclusions:

  • REVERSE offers a comprehensive, one-stop solution for rapid NGS data analysis in RNA selection/evolution studies.
  • The web server significantly reduces the computational burden, eliminating the need for specialized programming expertise.
  • REVERSE empowers researchers to efficiently extract meaningful insights from their experimental data.