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A Semi-quantitative Approach to Assess Biofilm Formation Using Wrinkled Colony Development

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Published on: June 7, 2012

Microbial Response to Micrometer-Scale Multiaxial Wrinkled Surfaces.

Luca Pellegrino¹, Lukas Simon Kriem², Eric S J Robles³

¹Department of Chemical Engineering, Imperial College London, London SW7 2AZ, United Kingdom.

ACS Applied Materials & Interfaces

|June 14, 2022

Summary

Micrometer-scale surface patterns, like checkerboard and herringbone, disrupt bacterial spatial arrangement and slow proliferation. Multiaxial patterning effectively delays early-stage bacterial growth on surfaces.

Keywords:

PDMS antibacterial antimicrobial patterning roughness surface topography wrinkling

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Assembly and Tracking of Microbial Community Development within a Microwell Array Platform

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Published on: June 6, 2017

Area of Science:

Biomaterials Science
Microbiology
Surface Science

Background:

Surface topography significantly influences microbial interactions.
Understanding how engineered surface patterns affect microbial behavior is crucial for controlling biofilms and infections.

Purpose of the Study:

To investigate the impact of micrometer-scale surface wrinkling patterns on bacterial and fungal attachment and proliferation.
To correlate microbial deformation and proliferation with specific surface pattern characteristics.

Main Methods:

Fabrication of sinusoidal, checkerboard, and herringbone patterns on polydimethylsiloxane (PDMS) via mechanical wrinkling.
Exposure of fabricated surfaces and flat controls to model bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli K12) and fungi (Candida albicans).
Analysis of microbial deformation, orientation, area coverage, and proliferation over time.

Main Results:

Microbial deformation and orientation correlated with surface pattern aspect ratio and local order.
Checkerboard and herringbone patterns disrupted bacterial spatial arrangement, impeding proliferation for several hours.
Bacterial proliferation showed linear scaling with available surface area, with significant reduction (∼50%) on patterned surfaces after initial delay.

Conclusions:

Micrometer-scale topography, particularly multiaxial patterning, significantly impacts microbial attachment and proliferation.
Engineered surface patterns offer a strategy to delay and frustrate early-stage bacterial proliferation.
A framework is proposed to rationalize the effects of surface topography on microbial action.