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Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
Published on: November 9, 2014
Maria A Lei1, Mariana Rivelli2, Alejandro M Iglesias2
1Universidad de Buenos Aires, Facultad de Odontología, Cátedra de Materiales Dentales, Buenos Aires, Argentina. alejandra.lei@odontologia.uba.ar.
This study compared how six different resin-based cements used for dental veneers change color after being exposed to simulated aging conditions. Researchers made small test samples of each cement and measured their color before and after two weeks of artificial aging. The aging process involved cycles of UV light and heat, followed by condensation. They found that the amount of color change varied between the cements. Panavia F2.0 showed the least change, while Resin Duo Cement showed the most. The results suggest that some cements are more stable in color over time than others. This information could help dentists choose materials that maintain their appearance longer.
Area of Science:
Background:
Color stability remains a major concern in dental restorations, particularly when using resin-based cements for veneers. Prior research has shown that these materials can undergo discoloration over time, but the extent of this change under accelerated aging conditions is not fully understood. No prior work had resolved the specific impact of different resin cement types on color stability after exposure to UV and thermal cycles. This gap motivated the need for a controlled experimental comparison of various commercially available cements. Understanding the factors that influence color change is essential for selecting materials that maintain aesthetic outcomes. The variability in cement composition and curing methods suggests that results may differ significantly between brands. This study aims to clarify the role of material type in long-term color stability. The findings could help clinicians make more informed choices about cement selection.
Purpose Of The Study:
This study aimed to evaluate the color change of six different resin-based cements after undergoing accelerated artificial aging. The motivation stemmed from the need to understand how these materials perform under simulated environmental stress. Clinicians require reliable data to choose cements that resist discoloration over time. The study focused on comparing the effects of aging on each material's color stability. No prior work had directly compared these specific brands under identical conditions. The experimental design allowed for a standardized assessment of color change. The goal was to identify which cements showed the least change after aging. The results could inform clinical recommendations for long-term aesthetic outcomes.
Main Methods:
The study involved fabricating cylindrical specimens from six different resin-based cements. Each material was tested in five replicates, resulting in 30 total specimens. Specimens were light-cured following manufacturer guidelines using a Coltolux LED unit. Initial color measurements were taken using an Easyshade Spectrophotometer. Specimens were then subjected to two weeks of accelerated artificial aging. The aging process included cycles of UV exposure and vapor condensation. Color was re-measured after the aging period to assess changes. The Vita scale and CIELAB values were used to quantify and compare color differences.
Main Results:
Color change was measured using the Vita scale and CIELAB values before and after aging. The mean color change on the Vita scale ranged from 2.00 for Panavia F2.0 to 10.00 for Resin Duo Cement. The Kruskal Wallis test showed significant differences between materials (p<0.05). Panavia F2.0 exhibited the smallest color change compared to all other cements. On the CIELAB scale, ΔE values ranged from 7.24 for Panavia F2.0 to 16.31 for Resin Duo Cement. ANOVA confirmed significant differences between materials (p<0.05). Tukey's test identified two distinct groups: PF and DC versus RX, PA, PC, and SO. The lowest standard deviation was observed in Panavia F2.0 and Resin Duo Cement. These results highlight the variability in color stability among resin-based cements.
Conclusions:
Under the experimental conditions of this study, accelerated aging significantly affected the color stability of the tested resin-based cements. The authors observed that color change varied significantly between materials (p<0.05). Panavia F2.0 showed the least color change compared to other cements. The results suggest that material type plays a key role in determining color stability. The study did not propose new mechanisms but confirmed the importance of material selection. The findings support the need for further research on long-term clinical performance. The authors did not make claims about future directions or drug targets. The study provides evidence that some cements maintain better aesthetic properties after aging.
Panavia F2.0 showed the smallest color change on both the Vita scale (2.00) and the CIELAB scale (7.24).
Color was measured using the Vita scale and CIELAB values before and after accelerated artificial aging.
The Kruskal Wallis test was used to compare color change across different cement types and identify significant differences.
The CIELAB scale provided a quantitative measure of color difference (ΔE) after aging, allowing for objective comparisons.
The aging process lasted two weeks, with 336 hours of alternating UV exposure and vapor condensation.
The authors concluded that accelerated aging significantly affects the color stability of resin-based cements.