Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Mechanism of Angiogenesis

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...

Regulation of Metabolism

Regulation of Metabolism

Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Synthesis of thiophene derived fluorescent sensor for Hg<sup>2+</sup> ion detection and its applications in cell imaging, latent fingerprint, and real water analysis.

Scientific reports·2025

Same author

Identification of 1-O-Galloyl -β-D-glucose as a potent activator of Sirtuin-1: an in-silico study.

Journal of molecular graphics & modelling·2025

Same author

From DNA to Big Data: NGS Technologies and Their Applications.

Methods in molecular biology (Clifton, N.J.)·2025

Same author

The translational impact of bioinformatics on traditional wet lab techniques.

Advances in pharmacology (San Diego, Calif.)·2025

Same author

Ethnic and region-specific genetic risk variants of stroke and its comorbid conditions can define the variations in the burden of stroke and its phenotypic traits.

eLife·2024

Same author

Genomic SNPs resolve the phylogeny of an ancient amphibian island radiation from the Seychelles.

Molecular phylogenetics and evolution·2024

Same journal

From Isoprene Units to Polyprenols and Dolichols: 70 Years of Polyisoprenoid Biosynthesis Research.

Cell biochemistry and biophysics·2026

Same journal

Therapeutic Insights into the Hepatoprotective Effect of Strobilanthes callosa.

Cell biochemistry and biophysics·2026

Same journal

The Dual Role of TGF-β and Hypoxia on MMP14-Mediated Invasion in PC3 Cells.

Cell biochemistry and biophysics·2026

Same journal

Microalgae-Mediated Synthesis of Gold Nanoparticles from Indonesian Chlorella vulgaris InaCC M205 with Potential Anticancer Properties for Biomedical Application.

Cell biochemistry and biophysics·2026

Same journal

Adsorption-driven Graphene Oxide SPR Biointerfaces: Coupled Thermodynamic, Kinetic, and Optical Modeling with Effective-layer Validation.

Cell biochemistry and biophysics·2026

Same journal

Identification of Ferroptosis-related Genes Associated with the Prognosis of Hepatocellular Carcinoma.

Cell biochemistry and biophysics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 8, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Metabolite Effect on Angiogenesis: Insights from Transcriptome Analysis.

P Sunitha¹, Kesavan R Arya¹, Achuthsankar S Nair¹

¹Department of Computational Biology and Bioinformatics, University of Kerala, Kariavattom, Thiruvananthapuram, 695581, Kerala, India.

Cell Biochemistry and Biophysics

|June 14, 2022

Summary

This summary is machine-generated.

Cellular metabolism, particularly glucose levels, significantly influences angiogenesis by altering genome-wide gene expression. This metabolic dependence is crucial for understanding tumor angiogenesis and developing targeted therapies.

Keywords:

Angiogenesis Differentially expressed genes Endothelial cells Glucose Metabolite VEGF responsive signature genes

More Related Videos

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Published on: August 3, 2018

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay

Published on: June 30, 2023

Related Experiment Videos

Last Updated: Sep 8, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Published on: August 3, 2018

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay

Published on: June 30, 2023

Area of Science:

Cell Biology
Molecular Biology
Metabolic Regulation

Background:

Cellular metabolic status impacts endothelial cell angiogenic potential.
Previous research linked metabolites like lactate and citrate to VEGFA-VEGFR2 signaling.
The effect of metabolic status on genome-wide gene expression in angiogenesis remains unclear.

Purpose of the Study:

To investigate how glucose metabolism affects genome-level gene expression in endothelial cells during angiogenesis.
To identify key genes and pathways modulated by glucose levels in angiogenesis.

Main Methods:

Computational analysis of transcriptome data from human umbilical vein endothelial cells (HUVECs) under varying glucose conditions (in vitro and in vivo).
Identification of differentially expressed genes, focusing on the VEGFA-VEGFR2 signaling pathway and VEGF signature genes.

Main Results:

Glucose metabolism significantly alters genome-level expression in endothelial cells, impacting angiogenesis.
Specific differentially expressed genes within the VEGFA-VEGFR2 pathway and VEGF signature genes were identified as critical hubs.
Demonstrated a clear dependence of angiogenesis on metabolite levels, especially glucose.

Conclusions:

Angiogenesis is metabolically dependent, with glucose availability playing a key regulatory role at the genomic level.
Understanding this glucose-mediated genomic modulation is vital for comprehending tumor angiogenesis.
Metabolic reprogramming in cancer cells critically influences angiogenesis, offering therapeutic targets.