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IGF2BP3 Worsens Lung Cancer through Modifying Long Non-coding RNA CERS6-AS1/microRNA-1202 Axis

An Yan1, Xiaowei Song2, Bao Liu1

  • 1Department of Thoracic Oncology, Harbin Medical University Cancer Hospital, Harbin 150000, Heilongjiang, China.

Current Medicinal Chemistry
|June 15, 2022
PubMed
Abstract

Insights

Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) promotes lung cancer by regulating the CERS6-AS1/miR-1202 axis and upregulating GDPD5. Inhibiting IGF2BP3 or targeting this axis may offer therapeutic strategies for lung cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) is implicated in epigenetic regulation of lung cancer.
  • The precise molecular mechanisms of IGF2BP3 in lung cancer progression require further elucidation.

Purpose of the Study:

  • To investigate the regulatory role of IGF2BP3 in lung cancer.
  • To explore the involvement of the long non-coding RNA CERS6-AS1/microRNA-1202 (CERS6-AS1/miR-1202) axis in IGF2BP3-mediated lung cancer development.

Main Methods:

  • Analysis of IGF2BP3, CERS6-AS1, miR-1202, and GDPD5 expression in clinical lung cancer samples.
  • Assessment of molecular interactions between IGF2BP3, CERS6-AS1, miR-1202, and GDPD5.
  • In vitro and in vivo functional assays using transfected lung cancer cells to evaluate the impact of modulating these molecules on tumor growth and progression.

Main Results:

  • Elevated levels of IGF2BP3, CERS6-AS1, and GDPD5, with decreased miR-1202, were observed in lung cancer tissues.
  • IGF2BP3 positively regulated CERS6-AS1, which in turn affected miR-1202 targeting of GDPD5.
  • Suppression of IGF2BP3 or CERS6-AS1, or overexpression of miR-1202, inhibited lung cancer progression and reversed IGF2BP3-induced effects.

Conclusions:

  • IGF2BP3 promotes lung cancer cell development by binding to the CERS6-AS1 promoter, leading to down-regulation of miR-1202.
  • This pathway may involve the upregulation of glycerophosphodiester phosphodiesterase domain containing 5 (GDPD5).
  • Targeting the IGF2BP3/CERS6-AS1/miR-1202/GDPD5 axis presents a potential therapeutic strategy for lung cancer.
Keywords:
Lung cancerglycerophosphodiester phosphodiesterase domain containing 5insulin-like growth factor 2long non-coding RNA CERS6- AS1mRNA-binding protein 3microRNA-1202

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