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Isolation and Characterization of Lytic Bacteriophages from Wastewater with Phage Therapy Potentials Against

Mona Khorshidtalab1, İnci Durukan1, Enis Fuat Tufekci2

  • 1Department of Medical Microbiology, Karadeniz Technical University Faculty of Medicine, Trabzon, Turkey.

The Eurasian Journal of Medicine
|June 15, 2022
PubMed
Abstract

Insights

Researchers isolated lytic bacteriophages from wastewater, demonstrating their potential for phage therapy against multidrug-resistant gram-negative bacteria. These phages showed broad host ranges and effective lytic activity.

Area of Science:

  • Microbiology
  • Bacteriology
  • Virology

Background:

  • Rising multidrug resistance (MDR) in bacteria necessitates novel therapeutic strategies.
  • Bacteriophages (phages) offer a promising alternative to conventional antibiotics.
  • Phage therapy involves using viruses that specifically infect and kill bacteria.

Purpose of the Study:

  • To isolate and characterize lytic bacteriophages from environmental sources.
  • To evaluate the lytic activity of isolated phages against gram-negative bacteria.
  • To assess the potential of these phages for therapeutic applications against MDR bacteria.

Main Methods:

  • Isolation of phages and host bacteria from wastewater samples.
  • Determination of lytic activity using the agar spot test.
  • Bacterial identification and antibiotic susceptibility testing (MALDI-TOF MS, Phoenix 100).
  • Morphological characterization of phages via transmission electron microscopy (TEM).
  • Genomic and burst size analysis of a broad-host-range phage (Enteroc21).

Main Results:

  • Multiple phages exhibited lytic activity against various gram-negative bacterial strains and species.
  • Phages were classified into Siphoviridae, Myoviridae, and Podoviridae families based on TEM.
  • The Enteroc21 phage possessed a large genome (>100 kb) and a burst size of 180 PFU/cell.
  • Several host strains displayed resistance to common antibiotics; Achromobacter xylosoxidans TRAX 13 was MDR.

Conclusions:

  • The isolated bacteriophages demonstrate significant lytic potential against diverse gram-negative bacteria.
  • These findings support the viability of using these phages as a therapeutic agent.
  • Phage therapy presents a viable strategy to combat infections caused by multidrug-resistant bacteria.

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