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Updated: Sep 8, 2025

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Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
Published on: May 14, 2020
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Immunostaining and Protein Pull-Down of Methyl-5-Cytosine-Marked RNA:DNA Hybrids
Haibo Yang1,2, Li Lan3,4
1Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown, MA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 15, 2022
Abstract:
RNA:DNA hybrids not only function in various physiological cellular processes but also represent a threat to genome integrity. The methyl-5-cytosine (m5C) marked RNA:DNA hybrids containing the m5C modified RNA strand add another layer of regulation to the R-loop structure. Here we describe the detection of m5C modifications in the context of RNA:DNA hybrids using immunostaining, and identification of specific binding partners of m5C marked RNA:DNA hybrids using pull-down method.

